Typhoid fever and genetic polymorphisms at the natural resistance-associated macrophage protein 1

Sarah J. Dunstan; Vo An Ho; Chau Minh Duc; Mai Ngoc Lanh; Cao Xuan Thanh Phuong; Christine Luxemburger; John Wain; Frank Dudbridge; Christopher S. Peacock; Deborah House; Christopher Parry; Tran Tinh Hien; Gordon Dougan; Jeremy Farrar; Jenefer M. Blackwell

doi:10.1086/319289

Typhoid fever and genetic polymorphisms at the natural resistance-associated macrophage protein 1

Sarah J. Dunstan, Vo An Ho, Chau Minh Duc, Mai Ngoc Lanh, Cao Xuan Thanh Phuong, Christine Luxemburger, John Wain, Frank Dudbridge, Christopher S. Peacock, Deborah House, Christopher Parry, Tran Tinh Hien, Gordon Dougan, Jeremy Farrar, Jenefer M. Blackwell

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Abstract

Control of Salmonella enterica serovar Typhimurium (S. typhimurium) infection in the mouse model of typhoid fever is critically dependent on the natural resistance-associated macrophage protein 1 (Nramp1). In this study, we examined the role of genetic polymorphisms in the human homologue, NRAMP1, in resistance to typhoid fever in southern Vietnam. Patients with blood-culture-confirmed typhoid fever and healthy control subjects were genotyped for 6 polymorphic markers within and near NRAMP1 on chromosome 2q35. Four single base-pair polymorphisms (274 C/T, 469+14 G/C, 1465-85 G/A, and D543N), a (GT)_nrepeat in the promoter region of NRAMP1 and D2S1471, and a microsatellite marker ∼130kb downstream of NRAMP1 were examined. The allelic and genotypic frequencies for each polymorphism were compared in case patients and control subjects. No allelic association was identified between the NRAMP1 alleles and typhoid fever susceptibility. In addition, neither homozygotes nor heterozygotes for any NRAMP1 variants were at increased risk of typhoid fever.

Original language	English
Pages (from-to)	1156-1160
Number of pages	5
Journal	The Journal of Infectious Diseases
Volume	183
Issue number	7
DOIs	https://doi.org/10.1086/319289
Publication status	Published - 1 Apr 2001
Externally published	Yes

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Dunstan, S. J., Ho, V. A., Duc, C. M., Lanh, M. N., Phuong, C. X. T., Luxemburger, C., Wain, J., Dudbridge, F., Peacock, C. S., House, D., Parry, C., Hien, T. T., Dougan, G., Farrar, J., & Blackwell, J. M. (2001). Typhoid fever and genetic polymorphisms at the natural resistance-associated macrophage protein 1. The Journal of Infectious Diseases, 183(7), 1156-1160. https://doi.org/10.1086/319289

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title = "Typhoid fever and genetic polymorphisms at the natural resistance-associated macrophage protein 1",

abstract = "Control of Salmonella enterica serovar Typhimurium (S. typhimurium) infection in the mouse model of typhoid fever is critically dependent on the natural resistance-associated macrophage protein 1 (Nramp1). In this study, we examined the role of genetic polymorphisms in the human homologue, NRAMP1, in resistance to typhoid fever in southern Vietnam. Patients with blood-culture-confirmed typhoid fever and healthy control subjects were genotyped for 6 polymorphic markers within and near NRAMP1 on chromosome 2q35. Four single base-pair polymorphisms (274 C/T, 469+14 G/C, 1465-85 G/A, and D543N), a (GT)nrepeat in the promoter region of NRAMP1 and D2S1471, and a microsatellite marker ∼130kb downstream of NRAMP1 were examined. The allelic and genotypic frequencies for each polymorphism were compared in case patients and control subjects. No allelic association was identified between the NRAMP1 alleles and typhoid fever susceptibility. In addition, neither homozygotes nor heterozygotes for any NRAMP1 variants were at increased risk of typhoid fever.",

author = "Dunstan, {Sarah J.} and Ho, {Vo An} and Duc, {Chau Minh} and Lanh, {Mai Ngoc} and Phuong, {Cao Xuan Thanh} and Christine Luxemburger and John Wain and Frank Dudbridge and Peacock, {Christopher S.} and Deborah House and Christopher Parry and Hien, {Tran Tinh} and Gordon Dougan and Jeremy Farrar and Blackwell, {Jenefer M.}",

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doi = "10.1086/319289",

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Dunstan, SJ, Ho, VA, Duc, CM, Lanh, MN, Phuong, CXT, Luxemburger, C, Wain, J, Dudbridge, F, Peacock, CS, House, D, Parry, C, Hien, TT, Dougan, G, Farrar, J & Blackwell, JM 2001, 'Typhoid fever and genetic polymorphisms at the natural resistance-associated macrophage protein 1', The Journal of Infectious Diseases, vol. 183, no. 7, pp. 1156-1160. https://doi.org/10.1086/319289

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AU - Dunstan, Sarah J.

AU - Ho, Vo An

AU - Duc, Chau Minh

AU - Lanh, Mai Ngoc

AU - Phuong, Cao Xuan Thanh

AU - Luxemburger, Christine

AU - Wain, John

AU - Dudbridge, Frank

AU - Peacock, Christopher S.

AU - House, Deborah

AU - Parry, Christopher

AU - Hien, Tran Tinh

AU - Dougan, Gordon

AU - Farrar, Jeremy

AU - Blackwell, Jenefer M.

PY - 2001/4/1

Y1 - 2001/4/1

N2 - Control of Salmonella enterica serovar Typhimurium (S. typhimurium) infection in the mouse model of typhoid fever is critically dependent on the natural resistance-associated macrophage protein 1 (Nramp1). In this study, we examined the role of genetic polymorphisms in the human homologue, NRAMP1, in resistance to typhoid fever in southern Vietnam. Patients with blood-culture-confirmed typhoid fever and healthy control subjects were genotyped for 6 polymorphic markers within and near NRAMP1 on chromosome 2q35. Four single base-pair polymorphisms (274 C/T, 469+14 G/C, 1465-85 G/A, and D543N), a (GT)nrepeat in the promoter region of NRAMP1 and D2S1471, and a microsatellite marker ∼130kb downstream of NRAMP1 were examined. The allelic and genotypic frequencies for each polymorphism were compared in case patients and control subjects. No allelic association was identified between the NRAMP1 alleles and typhoid fever susceptibility. In addition, neither homozygotes nor heterozygotes for any NRAMP1 variants were at increased risk of typhoid fever.

AB - Control of Salmonella enterica serovar Typhimurium (S. typhimurium) infection in the mouse model of typhoid fever is critically dependent on the natural resistance-associated macrophage protein 1 (Nramp1). In this study, we examined the role of genetic polymorphisms in the human homologue, NRAMP1, in resistance to typhoid fever in southern Vietnam. Patients with blood-culture-confirmed typhoid fever and healthy control subjects were genotyped for 6 polymorphic markers within and near NRAMP1 on chromosome 2q35. Four single base-pair polymorphisms (274 C/T, 469+14 G/C, 1465-85 G/A, and D543N), a (GT)nrepeat in the promoter region of NRAMP1 and D2S1471, and a microsatellite marker ∼130kb downstream of NRAMP1 were examined. The allelic and genotypic frequencies for each polymorphism were compared in case patients and control subjects. No allelic association was identified between the NRAMP1 alleles and typhoid fever susceptibility. In addition, neither homozygotes nor heterozygotes for any NRAMP1 variants were at increased risk of typhoid fever.

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JO - The Journal of Infectious Diseases

JF - The Journal of Infectious Diseases

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ER -

Typhoid fever and genetic polymorphisms at the natural resistance-associated macrophage protein 1

Abstract

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