TY - JOUR
T1 - Two new anti-rat macrophage monoclonal antibodies
AU - Kerr, Peter G.
AU - Li, Hai Ling
AU - Gadd, Stephen
AU - Atkins, Robert C.
PY - 1992/1/1
Y1 - 1992/1/1
N2 - There are relatively few monoclonal antibodies (MAbs) to rat monocyte/macrophages available. We describe here 2 new such antibodies. The first, 109.2, recognizes most rat monocyte/ macrophages and all polymorphs. The antigen recognized by this antibody is upregulated by 15 mins exposure to PMA (Phorbol myristate acetate) but down regulated by overnight exposure to LPS (lipopolysaccharide). It is probably an adhesion molecule and is likely to represent the rat equivalent of CD11b. The second antibody, 112.1, recognizes lysosyme in rat macrophages, particularly alveolar macrophages. In addition it also recognizes lysosyme in hen, rabbit and human macrophages. It also recognizes lysosyme in other tissues such as Paneth cells and proximal renal tubular cells.
AB - There are relatively few monoclonal antibodies (MAbs) to rat monocyte/macrophages available. We describe here 2 new such antibodies. The first, 109.2, recognizes most rat monocyte/ macrophages and all polymorphs. The antigen recognized by this antibody is upregulated by 15 mins exposure to PMA (Phorbol myristate acetate) but down regulated by overnight exposure to LPS (lipopolysaccharide). It is probably an adhesion molecule and is likely to represent the rat equivalent of CD11b. The second antibody, 112.1, recognizes lysosyme in rat macrophages, particularly alveolar macrophages. In addition it also recognizes lysosyme in hen, rabbit and human macrophages. It also recognizes lysosyme in other tissues such as Paneth cells and proximal renal tubular cells.
KW - Anti-rat macrophages
KW - Lysosyme
KW - Monoclonal antibodies
UR - http://www.scopus.com/inward/record.url?scp=0026650661&partnerID=8YFLogxK
U2 - 10.3109/00313029209063629
DO - 10.3109/00313029209063629
M3 - Article
C2 - 1641266
AN - SCOPUS:0026650661
SN - 0031-3025
VL - 24
SP - 80
EP - 86
JO - Pathology
JF - Pathology
IS - 2
ER -