Trichostatin A accentuates doxorubicin-induced hypertrophy in cardiac myocytes

Tom C. Karagiannis, Ann J E Lin, Katherine Ververis, Lisa Chang, Michelle M Tang, Jun Okabe, Assam El-Osta

Research output: Contribution to journalArticleResearchpeer-review

33 Citations (Scopus)

Abstract

Histone deacetylase inhibitors represent a new class of anticancer therapeutics and the expectation is that they will be most effective when used in combination with conventional cancer therapies, such as the anthracycline, doxorubicin. The dose-limiting side effect of doxorubicin is severe cardiotoxicity and evaluation of the effects of combinations of the anthracycline with histone deacetylase inhibitors in relevant models is important. We used a wellestablished in vitro model of doxorubicin-induced hypertrophy to examine the effects of the prototypical histone deacetylase inhibitor, Trichostatin A. Our findings indicate that doxorubicin modulates the expression of the hypertrophyassociated genes, ventricular myosin light chain-2, the alpha isoform of myosin heavy chain and atrial natriuretic peptide, an effect which is augmented by Trichostatin A. Furthermore, we show that Trichostatin A amplifies doxorubicin-induced DNA double strand breaks, as assessed by γH2AX formation. More generally, our findings highlight the importance of investigating potential side effects that may be associated with emerging combination therapies for cancer.

Original languageEnglish
Pages (from-to)659-668
Number of pages10
JournalAging
Volume2
Issue number10
Publication statusPublished - Oct 2010

Keywords

  • Cardiac differentiation
  • Cardiac hypertrophy
  • Cardiomyocyte
  • Doxorubicin
  • Histone deacetylase inhibitor
  • Trichostatin A

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