TRF1 is a critical trans-acting factor required for de novo telomere formation in human cells

Jun Okabe, Akiko Eguchi, Akinori Masago, Takao Hayakawa, Mahito Nakanishi

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7 Citations (Scopus)

Abstract

The duplex telomere repeat (TTAGGG)(n) is an essential cis-acting element of the mammalian telomere, and an exogenous telomere repeat can induce chromosome breakage and de novo telomere formation at the site of a break (telomere seeding). Telomere seeding requires the telomere repeat (TTAGGG)(n) more stringently than does an in vitro telomerase assay, suggesting that it reflects the activity of a critical trans-acting element of the functional telomere, in addition to telomerase. Furthermore, telomere seeding is induced at a frequency fluctuating widely among human cell lines, suggesting variation in the activity of this hypothetical factor among cells. In this study, we investigated the cellular factor(s) required for telomere formation using the frequency of telomere seeding as an index and identified TRF1, one of the telomere repeat binding proteins, as an essential trans-acting factor. The exogenous telomere repeat induces telomere formation at a frequency determined by the availability of TRF1, even in telomerase-negative cells. Our study shows clearly that TRF1 has a novel physiological significance distinct from its role as a regulator of telomere length in the endogenous chromosome. The possible role of TRF1 in cell aging and immortalization is discussed.

Original languageEnglish
Pages (from-to)2639-2650
Number of pages12
JournalHuman Molecular Genetics
Volume9
Issue number18
DOIs
Publication statusPublished - 1 Nov 2000
Externally publishedYes

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