Treatment of neonatal mice with Flt3 ligand leads to changes in dendritic cell subpopulations associated with enhanced IL-12 and IFN-α production

Sabine Vollstedt, Meredith O'Keeffe, Bernhard Odermatt, Ryf Beat, Bettina Glanzmann, Matthias Riesen, Ken Shortman, Mark Suter

Research output: Contribution to journalArticleResearchpeer-review

28 Citations (Scopus)

Abstract

Treatment with the hematopoietic growth factor Flt3 ligand (FL) increases DC numbers in neonatal mice and enhances their resistance against intracellular pathogens. Flow cytometric analysis showed the presence of conventional DC (cDC) and plasmacytoid pre-DC (pDC) in neonatal spleens from untreated and FL-treated mice. CD8α and MHC class II expression on cDC and pDC was higher on DC from FL-treated mice than on DC from control littermates. After FL treatment, two additional subpopulations of DC-lineage cells were found that were able to produce IL-12 and IFN-α. The IL-12 production of cDC from FL-treated animals was more than 50-fold increased and their ability to stimulate T cell proliferation was also increased. We conclude that the enhanced resistance against intracellular pathogens was due to increased numbers of DC-lineage cells and their increased ability to produce the essential cytokines.

Original languageEnglish
Pages (from-to)1849-1860
Number of pages12
JournalEuropean Journal of Immunology
Volume34
Issue number7
DOIs
Publication statusPublished - Jul 2004
Externally publishedYes

Keywords

  • Cytokine production
  • DC subpopulations
  • Flt3 ligand
  • Neonatal mice

Cite this