Treadmill running improves hindlimb arteriolar endothelial function in type 1 diabetic mice as visualized by X-ray microangiography

Takashi Sonobe, Hirotsugu Tsuchimochi, Daryl O Schwenke, James T Pearson, Mikiyasu Shirai

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BACKGROUND: Vascular function is impaired in patients with diabetes, however diabetic vascular dysfunction is ameliorated by exercise training. We aimed to clarify which hindlimb arterial segments are affected by treadmill running in the hindlimbs of streptozocin-induced type 1 diabetic mice in vivo. METHODS: Mice were divided into 3 groups; healthy control, diabetic control, and diabetic-running groups. The exercise regimen was performed by treadmill level running mice for 60 min/day, for 4 weeks. Thereafter, we examined the vascular response to systemic acetylcholine administration in the left hindlimb of anesthetized-ventilated mice using either 1) X-ray microangiography to visualize the arteries or 2) ultrasonic flowmetry to record the femoral arterial blood flow. RESULTS: X-ray imaging clearly visualized the hindlimb arterial network ( 70-250 mum diameter). The vasodilator response to acetylcholine was significantly attenuated locally in the arterioles
Original languageEnglish
Article number51
Number of pages10
JournalCardiovascular Diabetology
Issue number1
Publication statusPublished - 2015

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