Dietary phytochemicals promote health and reduce the risk of chronic disease. The Phytochemical Absorption Prediction Model (PCAP) predicts the time required for phytochemicals to reach maximal plasma concentrations (Tmax) in humans based on their lipophilicity and molecular mass. Cell-based transport models have been used to quantify transport rate and efficiency of pharmaceuticals and phytochemicals, however these parameters have not previously been related to the human absorption Tmax. Caco-2/HT29-MTX-E12 monolayers were used to characterise transport of phytochemical standards and extracts and to establish a relationship between the in vitro permeability (Papp) of standards and their in vivo Tmax predicted from the PCAP model. Lipophilic compounds transported through the cell monolayer at relatively faster rates (higher Papp) than hydrophilic compounds, whilst having slower predicted in vivo absorption rates (longer Tmax). The results infer differences between in vitro (cell monolayer) and in vivo (human gastrointestinal tract) absorption kinetics of phytochemicals.
|Number of pages||9|
|Journal||Journal of Functional Foods|
|Publication status||Published - 1 Dec 2017|
- log P
- Molecular mass
- Phytochemical absorption prediction
- Small intestine