Transplantation of human mesenchymal stem cells loaded on collagen scaffolds for the treatment of traumatic brain injury in rats

Jian-Guo Guan, Zhaohui Zhu, Robert Chunhua Zhao, Zhifeng Xiao, Chenxi Wu, Qin Han, Lei Chen, Weimin Tong, Jin Zhang, Qianqian Han, Jun Gao, Ming Feng, Xinjie Bao, Jianwu Dai, Renzhi Wang

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126 Citations (Scopus)


Studies have suggested that mesenchymal stem cells (MSCs) have therapeutic effects following traumatic brain injury (TBI). However, cell distribution and survival rate are two major barriers to their success as therapeutic treatment. The improvement of cell therapy using collagen delivery matrices had been reported. However, we know very little about the mechanisms. We labeled human bone marrow-derived mesenchymal stem cells (hMSCs) with a positron emission tomography (PET) tracer, 18F-fluoro-2-deoxy-d-glucose (FDG). hMSCs were transplanted with or without collagen scaffolds into rats with experimental TBI and the whole-body nuclear images were compared. Collagen scaffolds increased the retention of hBMSC in the lesion site and limited its distribution at the transplanted region. Significantly more hMSCs were detected in the brain when transplanted with collagen scaffolds. The results showed collagen scaffolds also efficiently improved cell survival and neurite outgrowth invivo, resulting in better neural functional recovery. In addition, brain metabolism also improved in the collagen scaffold implanted group, as evaluated by PET. We speculated that collagen scaffolds would improve early engraftment and support the survival of grafted cells post-transplantation.

Original languageEnglish
Pages (from-to)5937-5946
Number of pages10
Issue number24
Publication statusPublished - Aug 2013
Externally publishedYes


  • Biomaterials
  • Brain trauma
  • DAPI
  • Differentiation
  • ESCs
  • FDG
  • HMSCs
  • MSCs
  • Nuclear imaging
  • PET
  • ROI
  • Stem cells
  • TBI

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