Abstract
Transparent liquid marbles coated with hydrophobic silica nanoparticles were used as micro-bioreactors for embryonic stem cell (ESC) culturing. The high transparency of silica liquid marbles enables real-time and in situ monitoring of embryonic body (EB) formation and differentiation. The experimental result shows that ESCs can aggregate with each other close to the bottom of the liquid marble and form EBs, while remaining suspended in the culture media. The differentiation of the suspending EBs into contractile cardiomyocytes has been demonstrated inside the transparent liquid marbles, which enable the in situ microscopic observation. It was also found, through comparison, that ESCs in a bare sessile drop placed on a superhydrophobic substrate tend to anchor onto the substrate and then differentiate following the normal way of cell spreading, i.e., withdrawal from the cell cycle, fusion with nascent myotubes, and final differentiation into cardiomyocytes. In contrast, liquid marble particle shells weaken the adhesion of spherical EBs to the substrate, encouraging them to differentiate in suspension into cardiomyocytes, without anchoring. The results of this study highlight the promising performance of liquid marbles as "one-pot" micro-bioreactors for EB formation and differentiation.
| Original language | English |
|---|---|
| Pages (from-to) | 8789-8796 |
| Number of pages | 8 |
| Journal | ACS Applied Materials and Interfaces |
| Volume | 11 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 6 Mar 2019 |
Keywords
- "one-pot" differentiation
- embryonic bodies (EBs)
- embryonic stem cells (ESCs)
- nanoparticle-liquid interface
- transparent liquid marble
Cite this
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Transparent bioreactors based on nanoparticle-coated liquid marbles for in situ observation of suspending embryonic body formation and differentiation. / Lin, Kejun; Chen, Ruoyang; Zhang, Liyuan; Zang, Duyang; Geng, Xingguo; Shen, Wei.
In: ACS Applied Materials and Interfaces, Vol. 11, No. 9, 06.03.2019, p. 8789-8796.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Transparent bioreactors based on nanoparticle-coated liquid marbles for in situ observation of suspending embryonic body formation and differentiation
AU - Lin, Kejun
AU - Chen, Ruoyang
AU - Zhang, Liyuan
AU - Zang, Duyang
AU - Geng, Xingguo
AU - Shen, Wei
PY - 2019/3/6
Y1 - 2019/3/6
N2 - Transparent liquid marbles coated with hydrophobic silica nanoparticles were used as micro-bioreactors for embryonic stem cell (ESC) culturing. The high transparency of silica liquid marbles enables real-time and in situ monitoring of embryonic body (EB) formation and differentiation. The experimental result shows that ESCs can aggregate with each other close to the bottom of the liquid marble and form EBs, while remaining suspended in the culture media. The differentiation of the suspending EBs into contractile cardiomyocytes has been demonstrated inside the transparent liquid marbles, which enable the in situ microscopic observation. It was also found, through comparison, that ESCs in a bare sessile drop placed on a superhydrophobic substrate tend to anchor onto the substrate and then differentiate following the normal way of cell spreading, i.e., withdrawal from the cell cycle, fusion with nascent myotubes, and final differentiation into cardiomyocytes. In contrast, liquid marble particle shells weaken the adhesion of spherical EBs to the substrate, encouraging them to differentiate in suspension into cardiomyocytes, without anchoring. The results of this study highlight the promising performance of liquid marbles as "one-pot" micro-bioreactors for EB formation and differentiation.
AB - Transparent liquid marbles coated with hydrophobic silica nanoparticles were used as micro-bioreactors for embryonic stem cell (ESC) culturing. The high transparency of silica liquid marbles enables real-time and in situ monitoring of embryonic body (EB) formation and differentiation. The experimental result shows that ESCs can aggregate with each other close to the bottom of the liquid marble and form EBs, while remaining suspended in the culture media. The differentiation of the suspending EBs into contractile cardiomyocytes has been demonstrated inside the transparent liquid marbles, which enable the in situ microscopic observation. It was also found, through comparison, that ESCs in a bare sessile drop placed on a superhydrophobic substrate tend to anchor onto the substrate and then differentiate following the normal way of cell spreading, i.e., withdrawal from the cell cycle, fusion with nascent myotubes, and final differentiation into cardiomyocytes. In contrast, liquid marble particle shells weaken the adhesion of spherical EBs to the substrate, encouraging them to differentiate in suspension into cardiomyocytes, without anchoring. The results of this study highlight the promising performance of liquid marbles as "one-pot" micro-bioreactors for EB formation and differentiation.
KW - "one-pot" differentiation
KW - embryonic bodies (EBs)
KW - embryonic stem cells (ESCs)
KW - nanoparticle-liquid interface
KW - transparent liquid marble
UR - http://www.scopus.com/inward/record.url?scp=85062345387&partnerID=8YFLogxK
U2 - 10.1021/acsami.8b20169
DO - 10.1021/acsami.8b20169
M3 - Article
VL - 11
SP - 8789
EP - 8796
JO - ACS Applied Materials and Interfaces
JF - ACS Applied Materials and Interfaces
SN - 1944-8244
IS - 9
ER -