Transmission infrared spectroscopy of whole blood - complications for quantitative analysis from leucocyte adhesion during continuous monitoring

Thorsten Vahlsing, Uwe Damm, Venkata Radhakrishna Kondepati, Steffen Leonhardt, Mathias Brendel, Bayden Wood, Michael Heise

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11 Citations (Scopus)

Abstract

Infrared spectroscopy has been applied to analyse glucose and cellular components in whole blood with the aim of developing an online clinical diagnostic and monitoring modality. Leucocyte adsorption onto the CaF2 windows was observed over a period of several hours under continuous blood flow using a transmission cell of 30 I?m path length. This build-up of cellular material on the windows is responsible for diminishing the sample path length under the flow conditions chosen. The adsorption dynamics have been characterised and their impact on glucose monitoring is reported. For short-term monitoring (
Original languageEnglish
Pages (from-to)567 - 578
Number of pages12
JournalJournal of Biophotonics
Volume3
Issue number8-9
DOIs
Publication statusPublished - 2010

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