TY - JOUR
T1 - Transmembrane and trans-subunit regulation of ectodomain shedding of platelet glycoprotein Ibalpha
AU - Mo, Xi
AU - Nguyen, Nam X
AU - Mu, Fi -Tjen
AU - Yang, Wenjun
AU - Luo, Shi -Zhong
AU - Fan, Huizhou
AU - Andrews, Robert Keith
AU - Berndt, Michael Claude
AU - Li, Renhao
PY - 2010
Y1 - 2010
N2 - In this study, shedding of GPIba, the ligand-binding subunit of the platelet GPIb-IX complex and a marker for platelet senescence and storage lesion, was assessed in Chinese hamster ovary cells with/without functional GPIba sheddase ADAM17. Mutagenesis of the GPIb-IX complex, which contains GPIba, GPIbi??, and GPIX subunits, revealed that the intracellular membrane-proximal calmodulin-binding region of GPIbi?? is critical for ADAM17-dependent shedding of GPIba induced by the calmodulin inhibitor, W7.
AB - In this study, shedding of GPIba, the ligand-binding subunit of the platelet GPIb-IX complex and a marker for platelet senescence and storage lesion, was assessed in Chinese hamster ovary cells with/without functional GPIba sheddase ADAM17. Mutagenesis of the GPIb-IX complex, which contains GPIba, GPIbi??, and GPIX subunits, revealed that the intracellular membrane-proximal calmodulin-binding region of GPIbi?? is critical for ADAM17-dependent shedding of GPIba induced by the calmodulin inhibitor, W7.
UR - http://www.scopus.com/record/display.url?eid=2-s2.0-77957773114&origin=inward&txGid=K3AbOd1H4ccTSt5fvLaFPsG%3a19
U2 - 10.1074/jbc.M110.111864
DO - 10.1074/jbc.M110.111864
M3 - Article
VL - 285
SP - 32096
EP - 32104
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 1083-351X
IS - 42
ER -