Transmembrane and trans-subunit regulation of ectodomain shedding of platelet glycoprotein Ibalpha

Xi Mo, Nam X Nguyen, Fi -Tjen Mu, Wenjun Yang, Shi -Zhong Luo, Huizhou Fan, Robert Keith Andrews, Michael Claude Berndt, Renhao Li

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18 Citations (Scopus)

Abstract

In this study, shedding of GPIba, the ligand-binding subunit of the platelet GPIb-IX complex and a marker for platelet senescence and storage lesion, was assessed in Chinese hamster ovary cells with/without functional GPIba sheddase ADAM17. Mutagenesis of the GPIb-IX complex, which contains GPIba, GPIbi??, and GPIX subunits, revealed that the intracellular membrane-proximal calmodulin-binding region of GPIbi?? is critical for ADAM17-dependent shedding of GPIba induced by the calmodulin inhibitor, W7.
Original languageEnglish
Pages (from-to)32096 - 32104
Number of pages9
JournalJournal of Biological Chemistry
Volume285
Issue number42
DOIs
Publication statusPublished - 2010

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