Translational profiling: The genome-wide measure of the nascent proteome

Traude H. Beilharz, Thomas Preiss

Research output: Contribution to journalArticleResearchpeer-review

45 Citations (Scopus)

Abstract

Translation in eukaryotic cells is both physically and temporally separated from transcription. This provides cells with extended options to alter their proteome: (I) directly, by synchronising translation with an altering transcriptional profile; (2) by imposing a changed translational control over transcripts already present in the transcriptome; or (3) by a combination of (1) and (2). In this paper, recent findings in the controlled translation of the transcriptome using microarray analyses are reviewed. A guide to the current technologies and data analysis is also provided, and future directions in the study of translational control as the interface between the transcriptome and the proteome are outlined. This survey is focused on the yeast Saccharomyces cerevisiae, but the topics covered have universal relevance to the control of translation in eukaryotic cells.

Original languageEnglish
Pages (from-to)103-111
Number of pages9
JournalBriefings in Functional Genomics and Proteomics
Volume3
Issue number2
DOIs
Publication statusPublished - Aug 2004
Externally publishedYes

Keywords

  • Microarray
  • Mrna expression
  • Polysome profile
  • Proteome
  • Transcriptome
  • Translational control

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