Abstract
The EBV is known to persist in memory B cells, but it remains unclear how this affects cell numbers and humoral immunity. We here studied EBV persistence in memory B cell subsets and consequences on B cell memory in young children. EBV genome loads were quantified in 6 memory B cell subsets in EBV+ adults. The effects of EBV infection on memory B cell numbers and vaccination responses were studied longitudinally in children within the Generation R population cohort between 14 mo and 6 yr of age. EBV genomes were more numerous in CD27+ IgG+, CD27+ IgA+, and CD27-IgA+ memory B cells than in IgM-only, natural effector, and CD27-IgG+ B cells. The blood counts of IgM-only, CD27+IgA+, CD27-IgG+, and CD27+IgG+ memory B cells were significantly lower in EBV+ children than in uninfected controls at 14 mo of age—the age when these cells peak in numbers. At 6 yr, all of these memory B cell counts had normalized, as had plasma IgG levels to previous primary measles and booster tetanus vaccinations. In conclusion, EBV persists predominantly in Ig class-switched memory B cells, even when derived from T cell-independent responses (CD27-IgA+), and EBV infection results in a transient depletion of these cells in young children.
| Original language | English |
|---|---|
| Pages (from-to) | 949-956 |
| Number of pages | 8 |
| Journal | Journal of leukocyte biology |
| Volume | 101 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 1 Apr 2017 |
Keywords
- Epstein-Barr virus
- Herpes virus
- Humoral immunity
- Pediatric infection
Cite this
}
Transient reduction in IgA+ and IgG+ memory B cell numbers in young EBV-seropositive children : The generation R study. / van den Heuvel, Diana; Jansen, Michelle A E; Bell, Andrew I.; Rickinson, Alan B.; Jaddoe, Vincent W V; van Dongen, Jacques J M; Moll, Henriette A.; van Zelm, Menno C.
In: Journal of leukocyte biology, Vol. 101, No. 4, 01.04.2017, p. 949-956.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Transient reduction in IgA+ and IgG+ memory B cell numbers in young EBV-seropositive children
T2 - The generation R study
AU - van den Heuvel, Diana
AU - Jansen, Michelle A E
AU - Bell, Andrew I.
AU - Rickinson, Alan B.
AU - Jaddoe, Vincent W V
AU - van Dongen, Jacques J M
AU - Moll, Henriette A.
AU - van Zelm, Menno C.
PY - 2017/4/1
Y1 - 2017/4/1
N2 - The EBV is known to persist in memory B cells, but it remains unclear how this affects cell numbers and humoral immunity. We here studied EBV persistence in memory B cell subsets and consequences on B cell memory in young children. EBV genome loads were quantified in 6 memory B cell subsets in EBV+ adults. The effects of EBV infection on memory B cell numbers and vaccination responses were studied longitudinally in children within the Generation R population cohort between 14 mo and 6 yr of age. EBV genomes were more numerous in CD27+ IgG+, CD27+ IgA+, and CD27-IgA+ memory B cells than in IgM-only, natural effector, and CD27-IgG+ B cells. The blood counts of IgM-only, CD27+IgA+, CD27-IgG+, and CD27+IgG+ memory B cells were significantly lower in EBV+ children than in uninfected controls at 14 mo of age—the age when these cells peak in numbers. At 6 yr, all of these memory B cell counts had normalized, as had plasma IgG levels to previous primary measles and booster tetanus vaccinations. In conclusion, EBV persists predominantly in Ig class-switched memory B cells, even when derived from T cell-independent responses (CD27-IgA+), and EBV infection results in a transient depletion of these cells in young children.
AB - The EBV is known to persist in memory B cells, but it remains unclear how this affects cell numbers and humoral immunity. We here studied EBV persistence in memory B cell subsets and consequences on B cell memory in young children. EBV genome loads were quantified in 6 memory B cell subsets in EBV+ adults. The effects of EBV infection on memory B cell numbers and vaccination responses were studied longitudinally in children within the Generation R population cohort between 14 mo and 6 yr of age. EBV genomes were more numerous in CD27+ IgG+, CD27+ IgA+, and CD27-IgA+ memory B cells than in IgM-only, natural effector, and CD27-IgG+ B cells. The blood counts of IgM-only, CD27+IgA+, CD27-IgG+, and CD27+IgG+ memory B cells were significantly lower in EBV+ children than in uninfected controls at 14 mo of age—the age when these cells peak in numbers. At 6 yr, all of these memory B cell counts had normalized, as had plasma IgG levels to previous primary measles and booster tetanus vaccinations. In conclusion, EBV persists predominantly in Ig class-switched memory B cells, even when derived from T cell-independent responses (CD27-IgA+), and EBV infection results in a transient depletion of these cells in young children.
KW - Epstein-Barr virus
KW - Herpes virus
KW - Humoral immunity
KW - Pediatric infection
UR - http://www.scopus.com/inward/record.url?scp=85017329046&partnerID=8YFLogxK
U2 - 10.1189/jlb.5VMAB0616-283R
DO - 10.1189/jlb.5VMAB0616-283R
M3 - Article
VL - 101
SP - 949
EP - 956
JO - Journal of leukocyte biology
JF - Journal of leukocyte biology
SN - 0741-5400
IS - 4
ER -