Transient reduction in IgA+ and IgG+ memory B cell numbers in young EBV-seropositive children

The generation R study

Diana van den Heuvel, Michelle A E Jansen, Andrew I. Bell, Alan B. Rickinson, Vincent W V Jaddoe, Jacques J M van Dongen, Henriette A. Moll, Menno C. van Zelm

Research output: Contribution to journalArticleResearchpeer-review

2 Citations (Scopus)

Abstract

The EBV is known to persist in memory B cells, but it remains unclear how this affects cell numbers and humoral immunity. We here studied EBV persistence in memory B cell subsets and consequences on B cell memory in young children. EBV genome loads were quantified in 6 memory B cell subsets in EBV+ adults. The effects of EBV infection on memory B cell numbers and vaccination responses were studied longitudinally in children within the Generation R population cohort between 14 mo and 6 yr of age. EBV genomes were more numerous in CD27+ IgG+, CD27+ IgA+, and CD27-IgA+ memory B cells than in IgM-only, natural effector, and CD27-IgG+ B cells. The blood counts of IgM-only, CD27+IgA+, CD27-IgG+, and CD27+IgG+ memory B cells were significantly lower in EBV+ children than in uninfected controls at 14 mo of age—the age when these cells peak in numbers. At 6 yr, all of these memory B cell counts had normalized, as had plasma IgG levels to previous primary measles and booster tetanus vaccinations. In conclusion, EBV persists predominantly in Ig class-switched memory B cells, even when derived from T cell-independent responses (CD27-IgA+), and EBV infection results in a transient depletion of these cells in young children.

Original languageEnglish
Pages (from-to)949-956
Number of pages8
JournalJournal of leukocyte biology
Volume101
Issue number4
DOIs
Publication statusPublished - 1 Apr 2017

Keywords

  • Epstein-Barr virus
  • Herpes virus
  • Humoral immunity
  • Pediatric infection

Cite this

van den Heuvel, Diana ; Jansen, Michelle A E ; Bell, Andrew I. ; Rickinson, Alan B. ; Jaddoe, Vincent W V ; van Dongen, Jacques J M ; Moll, Henriette A. ; van Zelm, Menno C. / Transient reduction in IgA+ and IgG+ memory B cell numbers in young EBV-seropositive children : The generation R study. In: Journal of leukocyte biology. 2017 ; Vol. 101, No. 4. pp. 949-956.
@article{01734e7306784658b5f840562da473f5,
title = "Transient reduction in IgA+ and IgG+ memory B cell numbers in young EBV-seropositive children: The generation R study",
abstract = "The EBV is known to persist in memory B cells, but it remains unclear how this affects cell numbers and humoral immunity. We here studied EBV persistence in memory B cell subsets and consequences on B cell memory in young children. EBV genome loads were quantified in 6 memory B cell subsets in EBV+ adults. The effects of EBV infection on memory B cell numbers and vaccination responses were studied longitudinally in children within the Generation R population cohort between 14 mo and 6 yr of age. EBV genomes were more numerous in CD27+ IgG+, CD27+ IgA+, and CD27-IgA+ memory B cells than in IgM-only, natural effector, and CD27-IgG+ B cells. The blood counts of IgM-only, CD27+IgA+, CD27-IgG+, and CD27+IgG+ memory B cells were significantly lower in EBV+ children than in uninfected controls at 14 mo of age—the age when these cells peak in numbers. At 6 yr, all of these memory B cell counts had normalized, as had plasma IgG levels to previous primary measles and booster tetanus vaccinations. In conclusion, EBV persists predominantly in Ig class-switched memory B cells, even when derived from T cell-independent responses (CD27-IgA+), and EBV infection results in a transient depletion of these cells in young children.",
keywords = "Epstein-Barr virus, Herpes virus, Humoral immunity, Pediatric infection",
author = "{van den Heuvel}, Diana and Jansen, {Michelle A E} and Bell, {Andrew I.} and Rickinson, {Alan B.} and Jaddoe, {Vincent W V} and {van Dongen}, {Jacques J M} and Moll, {Henriette A.} and {van Zelm}, {Menno C.}",
year = "2017",
month = "4",
day = "1",
doi = "10.1189/jlb.5VMAB0616-283R",
language = "English",
volume = "101",
pages = "949--956",
journal = "Journal of leukocyte biology",
issn = "0741-5400",
publisher = "Society for Leukocyte Biology",
number = "4",

}

van den Heuvel, D, Jansen, MAE, Bell, AI, Rickinson, AB, Jaddoe, VWV, van Dongen, JJM, Moll, HA & van Zelm, MC 2017, 'Transient reduction in IgA+ and IgG+ memory B cell numbers in young EBV-seropositive children: The generation R study', Journal of leukocyte biology, vol. 101, no. 4, pp. 949-956. https://doi.org/10.1189/jlb.5VMAB0616-283R

Transient reduction in IgA+ and IgG+ memory B cell numbers in young EBV-seropositive children : The generation R study. / van den Heuvel, Diana; Jansen, Michelle A E; Bell, Andrew I.; Rickinson, Alan B.; Jaddoe, Vincent W V; van Dongen, Jacques J M; Moll, Henriette A.; van Zelm, Menno C.

In: Journal of leukocyte biology, Vol. 101, No. 4, 01.04.2017, p. 949-956.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Transient reduction in IgA+ and IgG+ memory B cell numbers in young EBV-seropositive children

T2 - The generation R study

AU - van den Heuvel, Diana

AU - Jansen, Michelle A E

AU - Bell, Andrew I.

AU - Rickinson, Alan B.

AU - Jaddoe, Vincent W V

AU - van Dongen, Jacques J M

AU - Moll, Henriette A.

AU - van Zelm, Menno C.

PY - 2017/4/1

Y1 - 2017/4/1

N2 - The EBV is known to persist in memory B cells, but it remains unclear how this affects cell numbers and humoral immunity. We here studied EBV persistence in memory B cell subsets and consequences on B cell memory in young children. EBV genome loads were quantified in 6 memory B cell subsets in EBV+ adults. The effects of EBV infection on memory B cell numbers and vaccination responses were studied longitudinally in children within the Generation R population cohort between 14 mo and 6 yr of age. EBV genomes were more numerous in CD27+ IgG+, CD27+ IgA+, and CD27-IgA+ memory B cells than in IgM-only, natural effector, and CD27-IgG+ B cells. The blood counts of IgM-only, CD27+IgA+, CD27-IgG+, and CD27+IgG+ memory B cells were significantly lower in EBV+ children than in uninfected controls at 14 mo of age—the age when these cells peak in numbers. At 6 yr, all of these memory B cell counts had normalized, as had plasma IgG levels to previous primary measles and booster tetanus vaccinations. In conclusion, EBV persists predominantly in Ig class-switched memory B cells, even when derived from T cell-independent responses (CD27-IgA+), and EBV infection results in a transient depletion of these cells in young children.

AB - The EBV is known to persist in memory B cells, but it remains unclear how this affects cell numbers and humoral immunity. We here studied EBV persistence in memory B cell subsets and consequences on B cell memory in young children. EBV genome loads were quantified in 6 memory B cell subsets in EBV+ adults. The effects of EBV infection on memory B cell numbers and vaccination responses were studied longitudinally in children within the Generation R population cohort between 14 mo and 6 yr of age. EBV genomes were more numerous in CD27+ IgG+, CD27+ IgA+, and CD27-IgA+ memory B cells than in IgM-only, natural effector, and CD27-IgG+ B cells. The blood counts of IgM-only, CD27+IgA+, CD27-IgG+, and CD27+IgG+ memory B cells were significantly lower in EBV+ children than in uninfected controls at 14 mo of age—the age when these cells peak in numbers. At 6 yr, all of these memory B cell counts had normalized, as had plasma IgG levels to previous primary measles and booster tetanus vaccinations. In conclusion, EBV persists predominantly in Ig class-switched memory B cells, even when derived from T cell-independent responses (CD27-IgA+), and EBV infection results in a transient depletion of these cells in young children.

KW - Epstein-Barr virus

KW - Herpes virus

KW - Humoral immunity

KW - Pediatric infection

UR - http://www.scopus.com/inward/record.url?scp=85017329046&partnerID=8YFLogxK

U2 - 10.1189/jlb.5VMAB0616-283R

DO - 10.1189/jlb.5VMAB0616-283R

M3 - Article

VL - 101

SP - 949

EP - 956

JO - Journal of leukocyte biology

JF - Journal of leukocyte biology

SN - 0741-5400

IS - 4

ER -