@article{1e5a78a111f842f2892be29deef18fd7,
title = "Transient introgression of Wolbachia into Aedes aegypti populations does not elicit an antibody response to Wolbachia surface protein in community members",
abstract = "Wolbachia is an endosymbiotic bacterium that can restrict the transmission of human pathogenic viruses by Aedes aegypti mosquitoes. Recent field trials have shown that dengue incidence is significantly reduced when Wolbachia is introgressed into the local Ae. aegypti population. Female Ae. aegypti are anautogenous and feed on human blood to produce viable eggs. Herein, we tested whether people who reside on Tri Nguyen Island (TNI), Vietnam developed antibodies to Wolbachia Surface Protein (WSP) following release of Wolbachia-infected Ae. aegypti, as a measure of exposure to Wolbachia. Paired blood samples were collected from 105 participants before and after mosquito releases and anti-WSP titres were measured by ELISA. We determined no change in anti-WSP titres after ~30 weeks of high levels of Wolbachia-Ae. aegypti on TNI. These data suggest that humans are not exposed to the major Wolbachia surface antigen, WSP, following introgression of Wolbachia-infected Ae. aegypti mosquitoes.",
keywords = "Aedes aegypti, arbovirus, dengue virus, vector biology, Wolbachia",
author = "Elvina Lee and Nguyen, {Tran Hien} and Nguyen, {Thu Yen} and Vu, {Sinh Nam} and Tran, {Nhu Duong} and Nghia, {Le Trung} and Vien, {Quang Mai} and Nguyen, {Thanh Dong} and Loterio, {Robson Kriiger} and I{\~n}aki Iturbe-Ormaetxe and Flores, {Heather A.} and O{\textquoteright}neill, {Scott L.} and Dang, {Duc Anh} and Simmons, {Cameron P.} and Fraser, {Johanna E.}",
note = "Funding Information: Funding: This research was funded by the Bill and Melinda Gates Foundation [OPP1180815], the Vector-Based Control of Transmission: Discovery Research (VCTR) program of the Grand Challenges in Global Health initiative [OPP1153619] managed by the Foundation for the National Institutes of Health, Welcome Trust [102591], and the Macquarie Group Foundation. Funding Information: Acknowledgments: We thank Brendon Chua (University of Melbourne, Australia) for gifting the tetanus toxoid protein. We acknowledge the Walter and Eliza Hall Institute Antibody Facility (Parkville, Melbourne, Australia) for the development of the rabbit polyclonal antibodies. Production and purification of Wolbachia Surface Protein was enabled by the National Biologics Facilities at the Commonwealth Scientific and Industrial Research Organisation (CSIRO; Parkville, Melbourne, Australia), which is supported by the National Collaborative Research Infrastructure Strategy and Therapeutic Innovation Australia. Publisher Copyright: {\textcopyright} 2022 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2022",
doi = "10.3390/pathogens11050535",
language = "English",
volume = "11",
journal = "Pathogens",
issn = "2076-0817",
publisher = "MDPI AG",
number = "5",
}
