Transgenic rice plants harboring the grain hardness-locus region of Aegilops tauschii

Go Suzuki, Hideo Wada, Hiromi Goto, Akiko Nakano, Haruna Oba, Takuya Deno, Sadequr Rahman, Yasuhiko Mukai

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    Grain hardness of wheat is determined by the hardness (Ha)-locus region, which contains three friabilin-related genes: puroindoline-a (Pina), puroindoline-b (Pinb) and GSP-1. In our previous study, we produced the transgenic rice plants harboring the large genomic fragment of the Ha-locus region of Aegilops tauschii containing Pina and GSP-1 genes by Agrobacterium-mediated transformation. To examine the effects of the transgenes in the rice endosperms, we firstly confirmed the homozygosity of the T-DNAs in four independent T 2 lines by using fluorescence in situ hybridization (FISH) and DNA gel blot analyses. The transgenes, Pina and GSP-1, were stably expressed in endosperms of the T 3 and T 4 seeds at RNA and protein levels, indicating that the promoters and other regulatory elements on the wheat Ha-locus region function in rice, and that multigene transformation using a large genomic fragment is a useful strategy. The functional contribution of the transgene-derived friabilins to the rice endosperm structure was considered as an increase of spaces between compound starch granules, resulting in a high proportion of white turbidity seeds.

    Original languageEnglish
    Pages (from-to)2293-2301
    Number of pages9
    JournalPlant Cell Reports
    Issue number12
    Publication statusPublished - Dec 2011


    • Compound starch granule
    • Friabilin
    • Grain hardness
    • Multigene transformation
    • Oryza sativa

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