TY - JOUR
T1 - Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance
AU - Madisen, Linda
AU - Garner, Aleena R.
AU - Shimaoka, Daisuke
AU - Chuong, Amy S.
AU - Klapoetke, Nathan C.
AU - Li, Lu
AU - van der Bourg, Alexander
AU - Niino, Yusuke
AU - Egolf, Ladan
AU - Monetti, Claudio
AU - Gu, Hong
AU - Mills, Maya
AU - Cheng, Adrian
AU - Tasic, Bosiljka
AU - Nguyen, Thuc Nghi
AU - Sunkin, Susan M.
AU - Benucci, Andrea
AU - Nagy, Andras
AU - Miyawaki, Atsushi
AU - Helmchen, Fritjof
AU - Empson, Ruth M.
AU - Knöpfel, Thomas
AU - Boyden, Edward S.
AU - Reid, R. Clay
AU - Carandini, Matteo
AU - Zeng, Hongkui
PY - 2015/3/4
Y1 - 2015/3/4
N2 - An increasingly powerful approach for studying brain circuits relies on targeting genetically encoded sensors and effectors to specific cell types. However, current approaches for this are still limited in functionality and specificity. Here we utilize several intersectional strategies to generate multiple transgenic mouse lines expressing high levels of novel genetic tools with high specificity. We developed driver and double reporter mouse lines and viral vectors using the Cre/Flp and Cre/Dre double recombinase systems and established a new, retargetable genomic locus, TIGRE, which allowed the generation of a large set of Cre/tTA-dependent reporter lines expressing fluorescent proteins, genetically encoded calcium, voltage, or glutamate indicators, and optogenetic effectors, all at substantially higher levels than before. High functionality was shown in example mouse lines for GCaMP6, YCX2.60, VSFP Butterfly 1.2, and Jaws. These novel transgenic lines greatly expand the ability to monitor and manipulate neuronal activities with increased specificity.
AB - An increasingly powerful approach for studying brain circuits relies on targeting genetically encoded sensors and effectors to specific cell types. However, current approaches for this are still limited in functionality and specificity. Here we utilize several intersectional strategies to generate multiple transgenic mouse lines expressing high levels of novel genetic tools with high specificity. We developed driver and double reporter mouse lines and viral vectors using the Cre/Flp and Cre/Dre double recombinase systems and established a new, retargetable genomic locus, TIGRE, which allowed the generation of a large set of Cre/tTA-dependent reporter lines expressing fluorescent proteins, genetically encoded calcium, voltage, or glutamate indicators, and optogenetic effectors, all at substantially higher levels than before. High functionality was shown in example mouse lines for GCaMP6, YCX2.60, VSFP Butterfly 1.2, and Jaws. These novel transgenic lines greatly expand the ability to monitor and manipulate neuronal activities with increased specificity.
UR - http://www.scopus.com/inward/record.url?scp=84924253852&partnerID=8YFLogxK
U2 - 10.1016/j.neuron.2015.02.022
DO - 10.1016/j.neuron.2015.02.022
M3 - Article
C2 - 25741722
AN - SCOPUS:84924253852
SN - 0896-6273
VL - 85
SP - 942
EP - 958
JO - Neuron
JF - Neuron
IS - 5
ER -