Transforming growth factor-β regulates tubular epithelial-myofibroblast transdifferentiation in vitro

Jun Ming Fan, N. G. Yee-Yung, Prudence A. Hill, David J. Nikolic-Paterson, Wei Mu, Robert C. Atkins, Hui Y. Lan

Research output: Contribution to journalArticleResearchpeer-review

441 Citations (Scopus)

Abstract

Background. We recently found evidence of tubular epithelial- myofibroblast transdifferentiation (TEMT) during the development of tubulointerstitial fibrosis in the rat remnant kidney. This study investigated the mechanisms that induce TEMT in vitro. Methods. The normal rat kidney tubular epithelial cell line (NRK52E) was cultured for six days on plastic or collagen type 1-coated plates in the presence or absence of recombinant transforming growth factor-β1 (TGF-β1). Transdifferentiation of tubular cells into myofibroblasts was assessed by electron microscopy and by expression of α-smooth muscle actin (α-SMA) and E-cadherin. Results. NRK52E cells cultured on plastic or collagen-coated plates showed a classic cobblestone morphology. Culture in 1 ng/ml TGF-β caused only very minor changes in morphology, but culture in 10 or 50 ng/ml TGF-β1 caused profound changes. This involved hypertrophy, a loss of apical-basal polarity and microvilli, with cells becoming elongated and invasive, the formation of a new front-end back-end polarity, and the appearance of actin microfilaments and dense bodies. These morphological changes were accompanied by phenotypic changes. Double immunohistochemistry staining showed that the addition of TGF-β1 to confluent cell cultures caused a loss of the epithelial marker E- cadherin and de novo expression of α-SMA. An intermediate stage in transdifferentiation could be seen with hypertrophic cells expressing both E- cadherin and α-SMA. De novo α-SMA expression was confirmed by Northern blotting, Western blotting, and flow cytometry. In particular, cells with a transformed morphology showed strong α-SMA immunostaining of characteristic microfilament structures along the cell axis. There was a dose-dependent increase in the percentage of cells expressing α-SMA with increasing concentrations of TGF-β1, which was completely inhibited by the addition of a neutralizing anti-TGF-β1 antibody. Compared with growth on plastic, cell culture on collagen-coated plates showed a threefold increase in the percentage of cells expressing α-SMA in response to TGF-β1. Conclusion. TGF-β1 is a key mediator that regulates, in a dose-dependent fashion, transdifferentiation of tubular epithelial cells into α-SMA+ myofibroblasts. This transdifferentiation is markedly enhanced by growth on collagen type I. These findings have identified a novel pathway that may contribute to renal fibrosis associated with overexpression of TGF-β1 within the diseased kidney.

Original languageEnglish
Pages (from-to)1455-1467
Number of pages13
JournalKidney International
Volume56
Issue number4
DOIs
Publication statusPublished - 1 Jan 1999

Keywords

  • Collagen type 1
  • Fibrosis
  • TGF-β
  • Transdifferentiation
  • Tubulointerstitial fibrosis
  • α-smooth muscle actin

Cite this

Fan, Jun Ming ; Yee-Yung, N. G. ; Hill, Prudence A. ; Nikolic-Paterson, David J. ; Mu, Wei ; Atkins, Robert C. ; Lan, Hui Y. / Transforming growth factor-β regulates tubular epithelial-myofibroblast transdifferentiation in vitro. In: Kidney International. 1999 ; Vol. 56, No. 4. pp. 1455-1467.
@article{550e3d4f21a54943a1e4c36a2e9a1946,
title = "Transforming growth factor-β regulates tubular epithelial-myofibroblast transdifferentiation in vitro",
abstract = "Background. We recently found evidence of tubular epithelial- myofibroblast transdifferentiation (TEMT) during the development of tubulointerstitial fibrosis in the rat remnant kidney. This study investigated the mechanisms that induce TEMT in vitro. Methods. The normal rat kidney tubular epithelial cell line (NRK52E) was cultured for six days on plastic or collagen type 1-coated plates in the presence or absence of recombinant transforming growth factor-β1 (TGF-β1). Transdifferentiation of tubular cells into myofibroblasts was assessed by electron microscopy and by expression of α-smooth muscle actin (α-SMA) and E-cadherin. Results. NRK52E cells cultured on plastic or collagen-coated plates showed a classic cobblestone morphology. Culture in 1 ng/ml TGF-β caused only very minor changes in morphology, but culture in 10 or 50 ng/ml TGF-β1 caused profound changes. This involved hypertrophy, a loss of apical-basal polarity and microvilli, with cells becoming elongated and invasive, the formation of a new front-end back-end polarity, and the appearance of actin microfilaments and dense bodies. These morphological changes were accompanied by phenotypic changes. Double immunohistochemistry staining showed that the addition of TGF-β1 to confluent cell cultures caused a loss of the epithelial marker E- cadherin and de novo expression of α-SMA. An intermediate stage in transdifferentiation could be seen with hypertrophic cells expressing both E- cadherin and α-SMA. De novo α-SMA expression was confirmed by Northern blotting, Western blotting, and flow cytometry. In particular, cells with a transformed morphology showed strong α-SMA immunostaining of characteristic microfilament structures along the cell axis. There was a dose-dependent increase in the percentage of cells expressing α-SMA with increasing concentrations of TGF-β1, which was completely inhibited by the addition of a neutralizing anti-TGF-β1 antibody. Compared with growth on plastic, cell culture on collagen-coated plates showed a threefold increase in the percentage of cells expressing α-SMA in response to TGF-β1. Conclusion. TGF-β1 is a key mediator that regulates, in a dose-dependent fashion, transdifferentiation of tubular epithelial cells into α-SMA+ myofibroblasts. This transdifferentiation is markedly enhanced by growth on collagen type I. These findings have identified a novel pathway that may contribute to renal fibrosis associated with overexpression of TGF-β1 within the diseased kidney.",
keywords = "Collagen type 1, Fibrosis, TGF-β, Transdifferentiation, Tubulointerstitial fibrosis, α-smooth muscle actin",
author = "Fan, {Jun Ming} and Yee-Yung, {N. G.} and Hill, {Prudence A.} and Nikolic-Paterson, {David J.} and Wei Mu and Atkins, {Robert C.} and Lan, {Hui Y.}",
year = "1999",
month = "1",
day = "1",
doi = "10.1046/j.1523-1755.1999.00656.x",
language = "English",
volume = "56",
pages = "1455--1467",
journal = "Kidney International",
issn = "0085-2538",
publisher = "Elsevier",
number = "4",

}

Transforming growth factor-β regulates tubular epithelial-myofibroblast transdifferentiation in vitro. / Fan, Jun Ming; Yee-Yung, N. G.; Hill, Prudence A.; Nikolic-Paterson, David J.; Mu, Wei; Atkins, Robert C.; Lan, Hui Y.

In: Kidney International, Vol. 56, No. 4, 01.01.1999, p. 1455-1467.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Transforming growth factor-β regulates tubular epithelial-myofibroblast transdifferentiation in vitro

AU - Fan, Jun Ming

AU - Yee-Yung, N. G.

AU - Hill, Prudence A.

AU - Nikolic-Paterson, David J.

AU - Mu, Wei

AU - Atkins, Robert C.

AU - Lan, Hui Y.

PY - 1999/1/1

Y1 - 1999/1/1

N2 - Background. We recently found evidence of tubular epithelial- myofibroblast transdifferentiation (TEMT) during the development of tubulointerstitial fibrosis in the rat remnant kidney. This study investigated the mechanisms that induce TEMT in vitro. Methods. The normal rat kidney tubular epithelial cell line (NRK52E) was cultured for six days on plastic or collagen type 1-coated plates in the presence or absence of recombinant transforming growth factor-β1 (TGF-β1). Transdifferentiation of tubular cells into myofibroblasts was assessed by electron microscopy and by expression of α-smooth muscle actin (α-SMA) and E-cadherin. Results. NRK52E cells cultured on plastic or collagen-coated plates showed a classic cobblestone morphology. Culture in 1 ng/ml TGF-β caused only very minor changes in morphology, but culture in 10 or 50 ng/ml TGF-β1 caused profound changes. This involved hypertrophy, a loss of apical-basal polarity and microvilli, with cells becoming elongated and invasive, the formation of a new front-end back-end polarity, and the appearance of actin microfilaments and dense bodies. These morphological changes were accompanied by phenotypic changes. Double immunohistochemistry staining showed that the addition of TGF-β1 to confluent cell cultures caused a loss of the epithelial marker E- cadherin and de novo expression of α-SMA. An intermediate stage in transdifferentiation could be seen with hypertrophic cells expressing both E- cadherin and α-SMA. De novo α-SMA expression was confirmed by Northern blotting, Western blotting, and flow cytometry. In particular, cells with a transformed morphology showed strong α-SMA immunostaining of characteristic microfilament structures along the cell axis. There was a dose-dependent increase in the percentage of cells expressing α-SMA with increasing concentrations of TGF-β1, which was completely inhibited by the addition of a neutralizing anti-TGF-β1 antibody. Compared with growth on plastic, cell culture on collagen-coated plates showed a threefold increase in the percentage of cells expressing α-SMA in response to TGF-β1. Conclusion. TGF-β1 is a key mediator that regulates, in a dose-dependent fashion, transdifferentiation of tubular epithelial cells into α-SMA+ myofibroblasts. This transdifferentiation is markedly enhanced by growth on collagen type I. These findings have identified a novel pathway that may contribute to renal fibrosis associated with overexpression of TGF-β1 within the diseased kidney.

AB - Background. We recently found evidence of tubular epithelial- myofibroblast transdifferentiation (TEMT) during the development of tubulointerstitial fibrosis in the rat remnant kidney. This study investigated the mechanisms that induce TEMT in vitro. Methods. The normal rat kidney tubular epithelial cell line (NRK52E) was cultured for six days on plastic or collagen type 1-coated plates in the presence or absence of recombinant transforming growth factor-β1 (TGF-β1). Transdifferentiation of tubular cells into myofibroblasts was assessed by electron microscopy and by expression of α-smooth muscle actin (α-SMA) and E-cadherin. Results. NRK52E cells cultured on plastic or collagen-coated plates showed a classic cobblestone morphology. Culture in 1 ng/ml TGF-β caused only very minor changes in morphology, but culture in 10 or 50 ng/ml TGF-β1 caused profound changes. This involved hypertrophy, a loss of apical-basal polarity and microvilli, with cells becoming elongated and invasive, the formation of a new front-end back-end polarity, and the appearance of actin microfilaments and dense bodies. These morphological changes were accompanied by phenotypic changes. Double immunohistochemistry staining showed that the addition of TGF-β1 to confluent cell cultures caused a loss of the epithelial marker E- cadherin and de novo expression of α-SMA. An intermediate stage in transdifferentiation could be seen with hypertrophic cells expressing both E- cadherin and α-SMA. De novo α-SMA expression was confirmed by Northern blotting, Western blotting, and flow cytometry. In particular, cells with a transformed morphology showed strong α-SMA immunostaining of characteristic microfilament structures along the cell axis. There was a dose-dependent increase in the percentage of cells expressing α-SMA with increasing concentrations of TGF-β1, which was completely inhibited by the addition of a neutralizing anti-TGF-β1 antibody. Compared with growth on plastic, cell culture on collagen-coated plates showed a threefold increase in the percentage of cells expressing α-SMA in response to TGF-β1. Conclusion. TGF-β1 is a key mediator that regulates, in a dose-dependent fashion, transdifferentiation of tubular epithelial cells into α-SMA+ myofibroblasts. This transdifferentiation is markedly enhanced by growth on collagen type I. These findings have identified a novel pathway that may contribute to renal fibrosis associated with overexpression of TGF-β1 within the diseased kidney.

KW - Collagen type 1

KW - Fibrosis

KW - TGF-β

KW - Transdifferentiation

KW - Tubulointerstitial fibrosis

KW - α-smooth muscle actin

UR - http://www.scopus.com/inward/record.url?scp=0032842887&partnerID=8YFLogxK

U2 - 10.1046/j.1523-1755.1999.00656.x

DO - 10.1046/j.1523-1755.1999.00656.x

M3 - Article

VL - 56

SP - 1455

EP - 1467

JO - Kidney International

JF - Kidney International

SN - 0085-2538

IS - 4

ER -