Transcriptome analysis of Drosophila melanogaster third instar larval ring glands points to novel functions and uncovers a cytochrome p450 required for development

Danielle Christesen, Ying Ting Yang, Jason Somers, Charles Robin, Tamar Sztal, Philip Batterham, Trent Perry

Research output: Contribution to journalArticleResearchpeer-review

10 Citations (Scopus)

Abstract

In Drosophila melanogaster larvae, the ring gland (RG) is a control center that orchestrates major developmental transitions. It is a composite organ, consisting of the prothoracic gland, the corpus allatum, and the corpora cardiaca, each of which synthesizes and secretes a different hormone. Until now, the RG's broader developmental roles beyond endocrine secretion have not been explored. RNA sequencing and analysis of a new transcriptome resource from D. melanogaster wandering third instar larval RGs has provided a fascinating insight into the diversity of developmental signaling in this organ. We have found strong enrichment of expression of two gene pathways not previously associated with the RG: immune response and fatty acid metabolism. We have also uncovered strong expression for many uncharacterized genes. Additionally, RNA interference against RG-enriched cytochrome p450s Cyp6u1 and Cyp6g2 produced a lethal ecdysone deficiency and a juvenile hormone deficiency, respectively, flagging a critical role for these genes in hormone synthesis. This transcriptome provides a valuable new resource for investigation of roles played by the RG in governing insect development.

Original languageEnglish
Pages (from-to)467-479
Number of pages13
JournalG3: Genes, Genomes, Genetics
Volume7
Issue number2
DOIs
Publication statusPublished - 2017

Keywords

  • Cytochrome p450
  • Ecdysteroidogenesis
  • Halloween genes
  • Immune response
  • Molting

Cite this

Christesen, Danielle ; Yang, Ying Ting ; Somers, Jason ; Robin, Charles ; Sztal, Tamar ; Batterham, Philip ; Perry, Trent. / Transcriptome analysis of Drosophila melanogaster third instar larval ring glands points to novel functions and uncovers a cytochrome p450 required for development. In: G3: Genes, Genomes, Genetics. 2017 ; Vol. 7, No. 2. pp. 467-479.
@article{51148dc32c1f4f98bf9895ad1e834e4e,
title = "Transcriptome analysis of Drosophila melanogaster third instar larval ring glands points to novel functions and uncovers a cytochrome p450 required for development",
abstract = "In Drosophila melanogaster larvae, the ring gland (RG) is a control center that orchestrates major developmental transitions. It is a composite organ, consisting of the prothoracic gland, the corpus allatum, and the corpora cardiaca, each of which synthesizes and secretes a different hormone. Until now, the RG's broader developmental roles beyond endocrine secretion have not been explored. RNA sequencing and analysis of a new transcriptome resource from D. melanogaster wandering third instar larval RGs has provided a fascinating insight into the diversity of developmental signaling in this organ. We have found strong enrichment of expression of two gene pathways not previously associated with the RG: immune response and fatty acid metabolism. We have also uncovered strong expression for many uncharacterized genes. Additionally, RNA interference against RG-enriched cytochrome p450s Cyp6u1 and Cyp6g2 produced a lethal ecdysone deficiency and a juvenile hormone deficiency, respectively, flagging a critical role for these genes in hormone synthesis. This transcriptome provides a valuable new resource for investigation of roles played by the RG in governing insect development.",
keywords = "Cytochrome p450, Ecdysteroidogenesis, Halloween genes, Immune response, Molting",
author = "Danielle Christesen and Yang, {Ying Ting} and Jason Somers and Charles Robin and Tamar Sztal and Philip Batterham and Trent Perry",
year = "2017",
doi = "10.1534/g3.116.037333",
language = "English",
volume = "7",
pages = "467--479",
journal = "G3: Genes, Genomes, Genetics",
issn = "2160-1836",
publisher = "Genetics Society of America",
number = "2",

}

Transcriptome analysis of Drosophila melanogaster third instar larval ring glands points to novel functions and uncovers a cytochrome p450 required for development. / Christesen, Danielle; Yang, Ying Ting; Somers, Jason; Robin, Charles; Sztal, Tamar; Batterham, Philip; Perry, Trent.

In: G3: Genes, Genomes, Genetics, Vol. 7, No. 2, 2017, p. 467-479.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Transcriptome analysis of Drosophila melanogaster third instar larval ring glands points to novel functions and uncovers a cytochrome p450 required for development

AU - Christesen, Danielle

AU - Yang, Ying Ting

AU - Somers, Jason

AU - Robin, Charles

AU - Sztal, Tamar

AU - Batterham, Philip

AU - Perry, Trent

PY - 2017

Y1 - 2017

N2 - In Drosophila melanogaster larvae, the ring gland (RG) is a control center that orchestrates major developmental transitions. It is a composite organ, consisting of the prothoracic gland, the corpus allatum, and the corpora cardiaca, each of which synthesizes and secretes a different hormone. Until now, the RG's broader developmental roles beyond endocrine secretion have not been explored. RNA sequencing and analysis of a new transcriptome resource from D. melanogaster wandering third instar larval RGs has provided a fascinating insight into the diversity of developmental signaling in this organ. We have found strong enrichment of expression of two gene pathways not previously associated with the RG: immune response and fatty acid metabolism. We have also uncovered strong expression for many uncharacterized genes. Additionally, RNA interference against RG-enriched cytochrome p450s Cyp6u1 and Cyp6g2 produced a lethal ecdysone deficiency and a juvenile hormone deficiency, respectively, flagging a critical role for these genes in hormone synthesis. This transcriptome provides a valuable new resource for investigation of roles played by the RG in governing insect development.

AB - In Drosophila melanogaster larvae, the ring gland (RG) is a control center that orchestrates major developmental transitions. It is a composite organ, consisting of the prothoracic gland, the corpus allatum, and the corpora cardiaca, each of which synthesizes and secretes a different hormone. Until now, the RG's broader developmental roles beyond endocrine secretion have not been explored. RNA sequencing and analysis of a new transcriptome resource from D. melanogaster wandering third instar larval RGs has provided a fascinating insight into the diversity of developmental signaling in this organ. We have found strong enrichment of expression of two gene pathways not previously associated with the RG: immune response and fatty acid metabolism. We have also uncovered strong expression for many uncharacterized genes. Additionally, RNA interference against RG-enriched cytochrome p450s Cyp6u1 and Cyp6g2 produced a lethal ecdysone deficiency and a juvenile hormone deficiency, respectively, flagging a critical role for these genes in hormone synthesis. This transcriptome provides a valuable new resource for investigation of roles played by the RG in governing insect development.

KW - Cytochrome p450

KW - Ecdysteroidogenesis

KW - Halloween genes

KW - Immune response

KW - Molting

UR - http://www.scopus.com/inward/record.url?scp=85012940938&partnerID=8YFLogxK

U2 - 10.1534/g3.116.037333

DO - 10.1534/g3.116.037333

M3 - Article

VL - 7

SP - 467

EP - 479

JO - G3: Genes, Genomes, Genetics

JF - G3: Genes, Genomes, Genetics

SN - 2160-1836

IS - 2

ER -