TY - JOUR
T1 - Transcriptome analysis during fruit developmental stages in durian (Durio zibethinus Murr.) var. D24
AU - Husin, Nurul Arneida
AU - Rahman, Sadequr
AU - Karunakaran, Rohini
AU - Bhore, Subhash Janardhan
N1 - Funding Information:
The authors want to acknowledge that the research funding for this project was provided by AIMST University, Malaysia, under its internal research grant scheme. The authors also want to thank the Agriculture Park of Universiti Putra Malaysia (UPM), Selangor, Malaysia, for providing the Durian (clone D24) fruit samples for the study.
Publisher Copyright:
© Sociedade Brasileira de Genética.
PY - 2022
Y1 - 2022
N2 - Durian (Durio zibethinus Murr.) fruits are famous for their unique aroma. This study analysed the Durian fruit transcriptome to discover the expression patterns of genes and to understand their regulation. Three developmental stages of Durian fruit, namely, early [90 days post-anthesis (DPA)], mature (120 DPA), and ripen (127 DPA), were studied. The Illumina HiSeq platform was used for sequencing. The sequence data were analysed using four different mapping aligners and statistical methods: CLC Genomic Workbench, HISAT2+DESeq2, Tophat+Cufflinks, and HISAT2+edgeR. The analyses showed that over 110 million clean reads were mapped to the Durian genome, yielding 19,976, 11,394, 17,833, and 24,351 differentially expressed genes during 90-127 days post-anthesis. Many identified differentially expressed genes were linked to the fruit ripening processes. The data analysis suggests that most genes with increased expression at the ripening stage were primarily involved in the metabolism of cofactors and vitamins, nucleotide metabolism, and carbohydrate metabolism. Significantly expressed genes from the young to mature stage were mainly associated with carbohydrate metabolism, amino acid metabolism, and cofactor and vitamin metabolism. The transcriptome data will serve as a foundation for understanding Durian fruit development-specific genes and could be helpful in fruit’s trait improvement.
AB - Durian (Durio zibethinus Murr.) fruits are famous for their unique aroma. This study analysed the Durian fruit transcriptome to discover the expression patterns of genes and to understand their regulation. Three developmental stages of Durian fruit, namely, early [90 days post-anthesis (DPA)], mature (120 DPA), and ripen (127 DPA), were studied. The Illumina HiSeq platform was used for sequencing. The sequence data were analysed using four different mapping aligners and statistical methods: CLC Genomic Workbench, HISAT2+DESeq2, Tophat+Cufflinks, and HISAT2+edgeR. The analyses showed that over 110 million clean reads were mapped to the Durian genome, yielding 19,976, 11,394, 17,833, and 24,351 differentially expressed genes during 90-127 days post-anthesis. Many identified differentially expressed genes were linked to the fruit ripening processes. The data analysis suggests that most genes with increased expression at the ripening stage were primarily involved in the metabolism of cofactors and vitamins, nucleotide metabolism, and carbohydrate metabolism. Significantly expressed genes from the young to mature stage were mainly associated with carbohydrate metabolism, amino acid metabolism, and cofactor and vitamin metabolism. The transcriptome data will serve as a foundation for understanding Durian fruit development-specific genes and could be helpful in fruit’s trait improvement.
KW - Durian
KW - Durio zibethinus
KW - ethylene
KW - fruit ripening
KW - transcriptome
UR - http://www.scopus.com/inward/record.url?scp=85146812028&partnerID=8YFLogxK
U2 - 10.1590/1678-4685-gmb-2021-0379
DO - 10.1590/1678-4685-gmb-2021-0379
M3 - Article
C2 - 36622241
AN - SCOPUS:85146812028
SN - 1415-4757
VL - 45
JO - Genetics and Molecular Biology
JF - Genetics and Molecular Biology
IS - 4
M1 - e20210379
ER -