Abstract
Human skin contains various populations of memory T cells in permanent residence and in transit. Arguably, the best characterized of the skin subsets are the CD8+ permanently resident memory T cells (TRM) expressing the integrin subunit, CD103. In order to investigate the remaining skin T cells, we isolated skin-tropic (CLA+) helper T cells, regulatory T cells, and CD8+ CD103- T cells from skin and blood for RNA microarray analysis to compare the transcriptional profiles of these groups.We found that despite their common tropism, the T cells isolated from skin were transcriptionally distinct from blood-derived CLA+ T cells. A shared pool of genes contributed to the skin/blood discrepancy, with substantial overlap in differentially expressed genes between each T cell subset. Gene set enrichment analysis further showed that the differential gene profiles of each human skin T cell subset were significantly enriched for previously identified TRM core signature genes. Our results support the hypothesis that human skin may contain additional TRM or TRM-like populations.
| Original language | English |
|---|---|
| Article number | 0148351 |
| Number of pages | 16 |
| Journal | PLoS ONE |
| Volume | 11 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 29 Jan 2016 |
| Externally published | Yes |
Keywords
- T cells
- cytotoxic T cells
- gene expression
- regulatory T cells
- blood
- skin
- memory T cells
- microarrays