Tracking protein aggregation and mislocalization in cells with flow cytometry

Yasmin M Ramdzan; Saskia Polling; Cheryl P Z Chia; Ivan Hong Wee Ng; Angelique R Ormsby; Nathan Paul Croft; Anthony Wayne Purcell; Marie Ann Bogoyevitch; Dominic CH Ng; Paul A Gleeson; Daniel Martin Hatters

doi:10.1038/nmeth.1930

Tracking protein aggregation and mislocalization in cells with flow cytometry

Yasmin M Ramdzan, Saskia Polling, Cheryl P Z Chia, Ivan Hong Wee Ng, Angelique R Ormsby, Nathan Paul Croft, Anthony Wayne Purcell, Marie Ann Bogoyevitch, Dominic CH Ng, Paul A Gleeson, Daniel Martin Hatters

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › Research › peer-review

94 Citations (Scopus)

Abstract

We applied pulse-shape analysis (PulSA) to monitor protein localization changes in mammalian cells by flow cytometry. PulSA enabled high-throughput tracking of protein aggregation, translocation from the cytoplasm to the nucleus and trafficking from the plasma membrane to the Golgi as well as stress-granule formation. Combining PulSA with tetracysteine-based oligomer sensors in a cell model of Huntington s disease enabled further separation of cells enriched with monomers, oligomers and inclusion bodies.

Original language	English
Pages (from-to)	467 - 470
Number of pages	4
Journal	Nature Methods
Volume	9
Issue number	5
DOIs	https://doi.org/10.1038/nmeth.1930
Publication status	Published - 2012

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10.1038/nmeth.1930

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title = "Tracking protein aggregation and mislocalization in cells with flow cytometry",

abstract = "We applied pulse-shape analysis (PulSA) to monitor protein localization changes in mammalian cells by flow cytometry. PulSA enabled high-throughput tracking of protein aggregation, translocation from the cytoplasm to the nucleus and trafficking from the plasma membrane to the Golgi as well as stress-granule formation. Combining PulSA with tetracysteine-based oligomer sensors in a cell model of Huntington s disease enabled further separation of cells enriched with monomers, oligomers and inclusion bodies.",

author = "Ramdzan, \{Yasmin M\} and Saskia Polling and Chia, \{Cheryl P Z\} and Ng, \{Ivan Hong Wee\} and Ormsby, \{Angelique R\} and Croft, \{Nathan Paul\} and Purcell, \{Anthony Wayne\} and Bogoyevitch, \{Marie Ann\} and Ng, \{Dominic CH\} and Gleeson, \{Paul A\} and Hatters, \{Daniel Martin\}",

year = "2012",

doi = "10.1038/nmeth.1930",

language = "English",

volume = "9",

pages = "467 -- 470",

journal = "Nature Methods",

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T1 - Tracking protein aggregation and mislocalization in cells with flow cytometry

AU - Ramdzan, Yasmin M

AU - Polling, Saskia

AU - Chia, Cheryl P Z

AU - Ng, Ivan Hong Wee

AU - Ormsby, Angelique R

AU - Croft, Nathan Paul

AU - Purcell, Anthony Wayne

AU - Bogoyevitch, Marie Ann

AU - Ng, Dominic CH

AU - Gleeson, Paul A

AU - Hatters, Daniel Martin

PY - 2012

Y1 - 2012

N2 - We applied pulse-shape analysis (PulSA) to monitor protein localization changes in mammalian cells by flow cytometry. PulSA enabled high-throughput tracking of protein aggregation, translocation from the cytoplasm to the nucleus and trafficking from the plasma membrane to the Golgi as well as stress-granule formation. Combining PulSA with tetracysteine-based oligomer sensors in a cell model of Huntington s disease enabled further separation of cells enriched with monomers, oligomers and inclusion bodies.

AB - We applied pulse-shape analysis (PulSA) to monitor protein localization changes in mammalian cells by flow cytometry. PulSA enabled high-throughput tracking of protein aggregation, translocation from the cytoplasm to the nucleus and trafficking from the plasma membrane to the Golgi as well as stress-granule formation. Combining PulSA with tetracysteine-based oligomer sensors in a cell model of Huntington s disease enabled further separation of cells enriched with monomers, oligomers and inclusion bodies.

UR - http://www.nature.com/nmeth/journal/v9/n5/pdf/nmeth.1930.pdf

UR - https://www.scopus.com/pages/publications/84860387931

U2 - 10.1038/nmeth.1930

DO - 10.1038/nmeth.1930

M3 - Article

SN - 1548-7091

VL - 9

SP - 467

EP - 470

JO - Nature Methods

JF - Nature Methods

IS - 5

ER -

Tracking protein aggregation and mislocalization in cells with flow cytometry

Abstract

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