Tracking protein aggregation and mislocalization in cells with flow cytometry

Yasmin M Ramdzan; Saskia Polling; Cheryl P Z Chia; Ivan Hong Wee Ng; Angelique R Ormsby; Nathan Paul Croft; Anthony Wayne Purcell; Marie Ann Bogoyevitch; Dominic CH Ng; Paul A Gleeson; Daniel Martin Hatters

doi:10.1038/nmeth.1930

Tracking protein aggregation and mislocalization in cells with flow cytometry

Yasmin M Ramdzan, Saskia Polling, Cheryl P Z Chia, Ivan Hong Wee Ng, Angelique R Ormsby, Nathan Paul Croft, Anthony Wayne Purcell, Marie Ann Bogoyevitch, Dominic CH Ng, Paul A Gleeson, Daniel Martin Hatters

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › Research › peer-review

56 Citations (Scopus)

Abstract

We applied pulse-shape analysis (PulSA) to monitor protein localization changes in mammalian cells by flow cytometry. PulSA enabled high-throughput tracking of protein aggregation, translocation from the cytoplasm to the nucleus and trafficking from the plasma membrane to the Golgi as well as stress-granule formation. Combining PulSA with tetracysteine-based oligomer sensors in a cell model of Huntington s disease enabled further separation of cells enriched with monomers, oligomers and inclusion bodies.

Original language	English
Pages (from-to)	467 - 470
Number of pages	4
Journal	Nature Methods
Volume	9
Issue number	5
DOIs	https://doi.org/10.1038/nmeth.1930
Publication status	Published - 2012

Cite this

Ramdzan, Y. M., Polling, S., Chia, C. P. Z., Ng, I. H. W., Ormsby, A. R., Croft, N. P., Purcell, A. W., Bogoyevitch, M. A., Ng, D. CH., Gleeson, P. A., & Hatters, D. M. (2012). Tracking protein aggregation and mislocalization in cells with flow cytometry. Nature Methods, 9(5), 467 - 470. https://doi.org/10.1038/nmeth.1930

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abstract = "We applied pulse-shape analysis (PulSA) to monitor protein localization changes in mammalian cells by flow cytometry. PulSA enabled high-throughput tracking of protein aggregation, translocation from the cytoplasm to the nucleus and trafficking from the plasma membrane to the Golgi as well as stress-granule formation. Combining PulSA with tetracysteine-based oligomer sensors in a cell model of Huntington s disease enabled further separation of cells enriched with monomers, oligomers and inclusion bodies.",

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doi = "10.1038/nmeth.1930",

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Tracking protein aggregation and mislocalization in cells with flow cytometry. / Ramdzan, Yasmin M; Polling, Saskia; Chia, Cheryl P Z; Ng, Ivan Hong Wee; Ormsby, Angelique R; Croft, Nathan Paul ; Purcell, Anthony Wayne; Bogoyevitch, Marie Ann; Ng, Dominic CH; Gleeson, Paul A; Hatters, Daniel Martin.

In: Nature Methods, Vol. 9, No. 5, 2012, p. 467 - 470.

Research output: Contribution to journal › Article › Research › peer-review

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AU - Chia, Cheryl P Z

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AU - Croft, Nathan Paul

AU - Purcell, Anthony Wayne

AU - Bogoyevitch, Marie Ann

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AU - Gleeson, Paul A

AU - Hatters, Daniel Martin

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