Tracking protein aggregation and mislocalization in cells with flow cytometry

Yasmin M Ramdzan; Saskia Polling; Cheryl P Z Chia; Ivan Hong Wee Ng; Angelique R Ormsby; Nathan Paul Croft; Anthony Wayne Purcell; Marie Ann Bogoyevitch; Dominic CH Ng; Paul A Gleeson; Daniel Martin Hatters

doi:10.1038/nmeth.1930

Tracking protein aggregation and mislocalization in cells with flow cytometry

Yasmin M Ramdzan, Saskia Polling, Cheryl P Z Chia, Ivan Hong Wee Ng, Angelique R Ormsby, Nathan Paul Croft, Anthony Wayne Purcell, Marie Ann Bogoyevitch, Dominic CH Ng, Paul A Gleeson, Daniel Martin Hatters

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › Research › peer-review

79 Citations (Scopus)

Abstract

We applied pulse-shape analysis (PulSA) to monitor protein localization changes in mammalian cells by flow cytometry. PulSA enabled high-throughput tracking of protein aggregation, translocation from the cytoplasm to the nucleus and trafficking from the plasma membrane to the Golgi as well as stress-granule formation. Combining PulSA with tetracysteine-based oligomer sensors in a cell model of Huntington s disease enabled further separation of cells enriched with monomers, oligomers and inclusion bodies.

Original language	English
Pages (from-to)	467 - 470
Number of pages	4
Journal	Nature Methods
Volume	9
Issue number	5
DOIs	https://doi.org/10.1038/nmeth.1930
Publication status	Published - 2012

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10.1038/nmeth.1930

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title = "Tracking protein aggregation and mislocalization in cells with flow cytometry",

abstract = "We applied pulse-shape analysis (PulSA) to monitor protein localization changes in mammalian cells by flow cytometry. PulSA enabled high-throughput tracking of protein aggregation, translocation from the cytoplasm to the nucleus and trafficking from the plasma membrane to the Golgi as well as stress-granule formation. Combining PulSA with tetracysteine-based oligomer sensors in a cell model of Huntington s disease enabled further separation of cells enriched with monomers, oligomers and inclusion bodies.",

author = "Ramdzan, {Yasmin M} and Saskia Polling and Chia, {Cheryl P Z} and Ng, {Ivan Hong Wee} and Ormsby, {Angelique R} and Croft, {Nathan Paul} and Purcell, {Anthony Wayne} and Bogoyevitch, {Marie Ann} and Ng, {Dominic CH} and Gleeson, {Paul A} and Hatters, {Daniel Martin}",

year = "2012",

doi = "10.1038/nmeth.1930",

language = "English",

volume = "9",

pages = "467 -- 470",

journal = "Nature Methods",

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Tracking protein aggregation and mislocalization in cells with flow cytometry. / Ramdzan, Yasmin M; Polling, Saskia; Chia, Cheryl P Z; Ng, Ivan Hong Wee; Ormsby, Angelique R; Croft, Nathan Paul ; Purcell, Anthony Wayne; Bogoyevitch, Marie Ann; Ng, Dominic CH; Gleeson, Paul A; Hatters, Daniel Martin.

In: Nature Methods, Vol. 9, No. 5, 2012, p. 467 - 470.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - Tracking protein aggregation and mislocalization in cells with flow cytometry

AU - Ramdzan, Yasmin M

AU - Polling, Saskia

AU - Chia, Cheryl P Z

AU - Ng, Ivan Hong Wee

AU - Ormsby, Angelique R

AU - Croft, Nathan Paul

AU - Purcell, Anthony Wayne

AU - Bogoyevitch, Marie Ann

AU - Ng, Dominic CH

AU - Gleeson, Paul A

AU - Hatters, Daniel Martin

PY - 2012

Y1 - 2012

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AB - We applied pulse-shape analysis (PulSA) to monitor protein localization changes in mammalian cells by flow cytometry. PulSA enabled high-throughput tracking of protein aggregation, translocation from the cytoplasm to the nucleus and trafficking from the plasma membrane to the Golgi as well as stress-granule formation. Combining PulSA with tetracysteine-based oligomer sensors in a cell model of Huntington s disease enabled further separation of cells enriched with monomers, oligomers and inclusion bodies.

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U2 - 10.1038/nmeth.1930

DO - 10.1038/nmeth.1930

M3 - Article

VL - 9

SP - 467

EP - 470

JO - Nature Methods

JF - Nature Methods

SN - 1548-7091

IS - 5

ER -

Tracking protein aggregation and mislocalization in cells with flow cytometry

Abstract

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