Abstract
We applied pulse-shape analysis (PulSA) to monitor protein localization changes in mammalian cells by flow cytometry. PulSA enabled high-throughput tracking of protein aggregation, translocation from the cytoplasm to the nucleus and trafficking from the plasma membrane to the Golgi as well as stress-granule formation. Combining PulSA with tetracysteine-based oligomer sensors in a cell model of Huntington s disease enabled further separation of cells enriched with monomers, oligomers and inclusion bodies.
Original language | English |
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Pages (from-to) | 467 - 470 |
Number of pages | 4 |
Journal | Nature Methods |
Volume | 9 |
Issue number | 5 |
DOIs | |
Publication status | Published - 2012 |