Tracking protein aggregation and mislocalization in cells with flow cytometry

Yasmin M Ramdzan, Saskia Polling, Cheryl P Z Chia, Ivan Hong Wee Ng, Angelique R Ormsby, Nathan Paul Croft, Anthony Wayne Purcell, Marie Ann Bogoyevitch, Dominic CH Ng, Paul A Gleeson, Daniel Martin Hatters

Research output: Contribution to journalArticleResearchpeer-review

56 Citations (Scopus)

Abstract

We applied pulse-shape analysis (PulSA) to monitor protein localization changes in mammalian cells by flow cytometry. PulSA enabled high-throughput tracking of protein aggregation, translocation from the cytoplasm to the nucleus and trafficking from the plasma membrane to the Golgi as well as stress-granule formation. Combining PulSA with tetracysteine-based oligomer sensors in a cell model of Huntington s disease enabled further separation of cells enriched with monomers, oligomers and inclusion bodies.
Original languageEnglish
Pages (from-to)467 - 470
Number of pages4
JournalNature Methods
Volume9
Issue number5
DOIs
Publication statusPublished - 2012

Cite this

Ramdzan, Y. M., Polling, S., Chia, C. P. Z., Ng, I. H. W., Ormsby, A. R., Croft, N. P., Purcell, A. W., Bogoyevitch, M. A., Ng, D. CH., Gleeson, P. A., & Hatters, D. M. (2012). Tracking protein aggregation and mislocalization in cells with flow cytometry. Nature Methods, 9(5), 467 - 470. https://doi.org/10.1038/nmeth.1930