Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 467 - 470 |
| Number of pages | 4 |
| Journal | Nature Methods |
| Volume | 9 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2012 |
Cite this
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Tracking protein aggregation and mislocalization in cells with flow cytometry. / Ramdzan, Yasmin M; Polling, Saskia; Chia, Cheryl P Z; Ng, Ivan Hong Wee; Ormsby, Angelique R; Croft, Nathan Paul; Purcell, Anthony Wayne; Bogoyevitch, Marie Ann; Ng, Dominic CH; Gleeson, Paul A; Hatters, Daniel Martin.
In: Nature Methods, Vol. 9, No. 5, 2012, p. 467 - 470.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Tracking protein aggregation and mislocalization in cells with flow cytometry
AU - Ramdzan, Yasmin M
AU - Polling, Saskia
AU - Chia, Cheryl P Z
AU - Ng, Ivan Hong Wee
AU - Ormsby, Angelique R
AU - Croft, Nathan Paul
AU - Purcell, Anthony Wayne
AU - Bogoyevitch, Marie Ann
AU - Ng, Dominic CH
AU - Gleeson, Paul A
AU - Hatters, Daniel Martin
PY - 2012
Y1 - 2012
N2 - We applied pulse-shape analysis (PulSA) to monitor protein localization changes in mammalian cells by flow cytometry. PulSA enabled high-throughput tracking of protein aggregation, translocation from the cytoplasm to the nucleus and trafficking from the plasma membrane to the Golgi as well as stress-granule formation. Combining PulSA with tetracysteine-based oligomer sensors in a cell model of Huntington s disease enabled further separation of cells enriched with monomers, oligomers and inclusion bodies.
AB - We applied pulse-shape analysis (PulSA) to monitor protein localization changes in mammalian cells by flow cytometry. PulSA enabled high-throughput tracking of protein aggregation, translocation from the cytoplasm to the nucleus and trafficking from the plasma membrane to the Golgi as well as stress-granule formation. Combining PulSA with tetracysteine-based oligomer sensors in a cell model of Huntington s disease enabled further separation of cells enriched with monomers, oligomers and inclusion bodies.
UR - http://www.nature.com/nmeth/journal/v9/n5/pdf/nmeth.1930.pdf
U2 - 10.1038/nmeth.1930
DO - 10.1038/nmeth.1930
M3 - Article
VL - 9
SP - 467
EP - 470
JO - Nature Methods
JF - Nature Methods
SN - 1548-7091
IS - 5
ER -