We applied pulse-shape analysis (PulSA) to monitor protein localization changes in mammalian cells by flow cytometry. PulSA enabled high-throughput tracking of protein aggregation, translocation from the cytoplasm to the nucleus and trafficking from the plasma membrane to the Golgi as well as stress-granule formation. Combining PulSA with tetracysteine-based oligomer sensors in a cell model of Huntington s disease enabled further separation of cells enriched with monomers, oligomers and inclusion bodies.
Ramdzan, Y. M., Polling, S., Chia, C. P. Z., Ng, I. H. W., Ormsby, A. R., Croft, N. P., Purcell, A. W.
, Bogoyevitch, M. A., Ng, D. CH., Gleeson, P. A., & Hatters, D. M. (2012). Tracking protein aggregation and mislocalization in cells with flow cytometry
. Nature Methods
(5), 467 - 470. https://doi.org/10.1038/nmeth.1930