Toxinology of venoms from five Australian lesser known elapid snakes

Kyle Pycroft, Bryan G Fry, Geoffrey K Isbister, D Sanjaya M Kuruppu, Josie Michelle Lawrence, Alexander Ian Smith, Wayne Clarence Hodgson

Research output: Contribution to journalArticleResearchpeer-review

11 Citations (Scopus)

Abstract

Research into Australian elapid venoms has mainly focused on the seven genera of greatest clinical significance: Acanthophis, Hoplocephalus, Notechis, Oxyuranus, Pseudechis, Pseudonaja and Tropidechis. However, even small species represent a potential for causing severe clinical envenoming. Further, owing to taxonomic distinctiveness, these species are a potential source of novel toxins for use in drug design and development. This is the first study to characterize the venoms of Cryptophis boschmai, Denisonia devisi, Echiopsis curta, Hemiaspis signata and Vermicella annulata. MALDI analysis of each venom, over the range of 4-40 kDa, indicated components in the weight range for three finger toxins (6-8 kDa) and phospholipase A(2) (PLA(2) ; 12-14 kDA). Interestingly, C. boschmai venom was the only venom, which contained components > 25 kDa. All venoms (10 mug/ml) demonstrated in vitro neurotoxicity in the chick biventer cervicis nerve-muscle preparation, with a relative rank order of: H. signata >/= D. devisi >/= V. annulata = E. curta > C. boschmai. CSL polyvalent antivenom neutralized the inhibitory effects of C. boschmai venom but only delayed the inhibitory effect of the other venoms. All venoms displayed PLA(2) activity but over a wide range (i.e. 1-621 mumol/min./mg). The venoms of C. boschmai (60 mug/kg, i.v.), D. devisi (60 mug/kg, i.v.) and H. signata (60 mug/kg, i.v.) produced hypotensive effects in vivo in an anaesthetized rat preparation. H. signata displayed moderate pro-coagulant activity while the other venoms were weakly pro-coagulant. This study demonstrated that these understudied Australian elapids have varying pharmacological activity, with notable in vitro neurotoxicity for four of the venoms, and may produce mild to moderate effects following systemic envenoming.
Original languageEnglish
Pages (from-to)268 - 274
Number of pages7
JournalBasic & Clinical Pharmacology & Toxicology
Volume111
Issue number4
DOIs
Publication statusPublished - 2012

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