Toxicity of aqueous C 70-gallic acid suspension in Daphnia magna

Brandon C. Seda, Pu Chun Ke, Andrew S. Mount, Stephen J. Klaine

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19 Citations (Scopus)


The present study assessed the toxic effects of stable aqueous colloidal suspensions of gallic-acid-stabilized C 70 fullerene on Daphnia magna. The suspensions were stabilized through noncovalent surface modification with gallic acid. In addition to whole-organism responses, changes in antioxidative processes in D. magna were quantified. Acute toxicity was observed with 96LC50 for C 70-gallic acid of 0.4±0.1mg/LC 70. Daphnia magna fecundity was significantly reduced in 21-d bioassays at C 70-gallic aqcid concentrations below quantifiable limits. Antioxidant enzyme activities of glutathione peroxidase and superoxide dismutase as well as lipid peroxidation suggested that exposed organisms experienced oxidative stress. Microscopic techniques used to determine cellular toxicity via apoptosis proved unsuccessful.

Original languageEnglish
Pages (from-to)215-220
Number of pages6
JournalEnvironmental Toxicology and Chemistry
Issue number1
Publication statusPublished - Jan 2012
Externally publishedYes


  • Colloids
  • Daphnia magna
  • Fullerene
  • Nanoparticles
  • Toxicity

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