Toxic town: ART disinfectants damage human sperms: An investigation into potential toxicity of ART disinfectants on human sperms

Sibgha Fatima, Zanib Arif, Humaira Gulnaz, Nabeela Kaukab, Sally Catt

Research output: Contribution to journalArticleResearch

Abstract

Aim: To investigate toxicity of disinfectants using human sperm motility assay (HSMA) in order to establish universal disinfection protocol, safe by all means for ART set up. Methods: Semen samples obtained from normospermic men and processed through density gradient, pellet was resuspended in 5ml of KSOM handling medium and motility assessed at 0 hours. The replicate splitted equally into five 1ml aliquots.In direct contact trial, four test tubes treated by adding 0.5ml of each disinfectant simultaneously (70% Ethanol, Fertisafe, Fertisafe Plus & Farmacidal) directly, one test tube without disinfectant being a control. Results: Human sperm motility was lost after 24hrs of direct contact trial treatment with disinfectants as compared to control (p<0.01). Sperm motility lost was more than 90 percent after 24hrs of direct contacttrial of disinfectants. Conclusion: Significant association of disinfectant use in ART setup and loss of sperm motility was found in direct contacttrial.

Original languageEnglish
Pages (from-to)1413-1415
Number of pages3
JournalPakistan Journal of Medical and Health Sciences
Volume12
Issue number4
Publication statusPublished - 1 Jan 2018

Keywords

  • Assistedreproductive technologies (ART)
  • Disinfectant
  • Normospermic
  • Sperm motility
  • Toxicity

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