TY - JOUR
T1 - Towards proteomics-on-chip
T2 - The role of the surface
AU - Salim, Malinda
AU - McArthur, Sally Louise
AU - Vaidyanathan, Seetharaman
AU - Wright, Phillip C.
PY - 2011/1/1
Y1 - 2011/1/1
N2 - Miniaturisation is revolutionary to high-throughput proteomics. These technologies have gained much interest in the past decade, as they allow for sensitive parallel analysis of small amounts of biological materials. This review describes the state of the art of proteomics-on-chip, with a particular focus on the fundamental proteomics-on-chip challenges. The important role of bio-interfacial interactions and strategies to control them are presented. Various coating methodologies for on-chip protein/peptide separation are reviewed to provide an overview of the principles of protein-resistant and protein immobilisation coatings, and their effectiveness.
AB - Miniaturisation is revolutionary to high-throughput proteomics. These technologies have gained much interest in the past decade, as they allow for sensitive parallel analysis of small amounts of biological materials. This review describes the state of the art of proteomics-on-chip, with a particular focus on the fundamental proteomics-on-chip challenges. The important role of bio-interfacial interactions and strategies to control them are presented. Various coating methodologies for on-chip protein/peptide separation are reviewed to provide an overview of the principles of protein-resistant and protein immobilisation coatings, and their effectiveness.
UR - http://www.scopus.com/inward/record.url?scp=78650086304&partnerID=8YFLogxK
U2 - 10.1039/c005236a
DO - 10.1039/c005236a
M3 - Review Article
C2 - 20714507
AN - SCOPUS:78650086304
SN - 1742-206X
VL - 7
SP - 101
EP - 115
JO - Molecular BioSystems
JF - Molecular BioSystems
IS - 1
ER -