Towards high throughput screening for drug discovery in multi splitting and merging system using microvalves

Single-layer microvalves are biocompatible, simple, and can be used at high-speed. Here, by the integration of these microvalves with other droplet microfluidic manipulation methods, we unveil an easy technique to use for splitting and merging of microdroplets at multiple locations, which makes it ideal for closed channel microfluidic high throughput screening. The presented multi splitting and merging microfluidic system is capable of generating four different kinds of droplets using four T-junctions, split them selectively in different volumes at four junctions using microvalves and merge the same/different kind of split droplets in various concentrations using pillar induced merging method.