Towards best practice in establishing patient-derived xenografts

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

Abstract

It is remarkable that human tumour tissues can be grown for months, or even years, as serially transplantable, patient-derived xenografts (PDXs) in immunocompromised mice. The grafting technique used has been refined over the last few decades, so it is now possible to successfully engraft most tumour types, albeit with varying take rates. This review focuses on the methodological requirements to establish successful PDXs. The first step is selecting viable tumour tissue from surgical resections of local or metastatic disease, ascites, pleural effusions, biopsies, circulating tumour cells, rapid autopsies or even organoids. Once grafts are prepared, sometimes with Matrigel or stroma, their likelihood of growing is affected by the strain of immunocompromised host mice and the graft site, which may be subcutaneous, subrenal capsule or orthotopic. Finally, once PDXs are established, authentication assays can be used to rule out possible misidentification or cross-contamination following serial passaging. By carefully optimising all of these steps, PDXs can be grown as efficiently as possible, providing invaluable models for preclinical cancer research.
Original languageEnglish
Title of host publicationPatient-Derived Xenograft Models of Human Cancer
EditorsYuzhuo Wang, Dong Lin, Peter W. Gout
PublisherSpringer
Pages11-28
Number of pages18
Edition1st
ISBN (Electronic)9783319558257
ISBN (Print)9783319558240
DOIs
Publication statusPublished - 30 Jun 2017

Keywords

  • patient-derived xenograft
  • tumour graft
  • metastasis
  • subcutaneous
  • renal capsule
  • orthotopic
  • immunocompromised mice
  • prostate cancer
  • breast cancer

Cite this

Risbridger, G. P., & Lawrence, M. G. (2017). Towards best practice in establishing patient-derived xenografts. In Y. Wang, D. Lin, & P. W. Gout (Eds.), Patient-Derived Xenograft Models of Human Cancer (1st ed., pp. 11-28). Springer. https://doi.org/10.1007/978-3-319-55825-7_2
Risbridger, Gail P. ; Lawrence, Mitchell G. / Towards best practice in establishing patient-derived xenografts. Patient-Derived Xenograft Models of Human Cancer. editor / Yuzhuo Wang ; Dong Lin ; Peter W. Gout. 1st. ed. Springer, 2017. pp. 11-28
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Risbridger, GP & Lawrence, MG 2017, Towards best practice in establishing patient-derived xenografts. in Y Wang, D Lin & PW Gout (eds), Patient-Derived Xenograft Models of Human Cancer. 1st edn, Springer, pp. 11-28. https://doi.org/10.1007/978-3-319-55825-7_2

Towards best practice in establishing patient-derived xenografts. / Risbridger, Gail P.; Lawrence, Mitchell G.

Patient-Derived Xenograft Models of Human Cancer. ed. / Yuzhuo Wang; Dong Lin; Peter W. Gout. 1st. ed. Springer, 2017. p. 11-28.

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

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AB - It is remarkable that human tumour tissues can be grown for months, or even years, as serially transplantable, patient-derived xenografts (PDXs) in immunocompromised mice. The grafting technique used has been refined over the last few decades, so it is now possible to successfully engraft most tumour types, albeit with varying take rates. This review focuses on the methodological requirements to establish successful PDXs. The first step is selecting viable tumour tissue from surgical resections of local or metastatic disease, ascites, pleural effusions, biopsies, circulating tumour cells, rapid autopsies or even organoids. Once grafts are prepared, sometimes with Matrigel or stroma, their likelihood of growing is affected by the strain of immunocompromised host mice and the graft site, which may be subcutaneous, subrenal capsule or orthotopic. Finally, once PDXs are established, authentication assays can be used to rule out possible misidentification or cross-contamination following serial passaging. By carefully optimising all of these steps, PDXs can be grown as efficiently as possible, providing invaluable models for preclinical cancer research.

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Risbridger GP, Lawrence MG. Towards best practice in establishing patient-derived xenografts. In Wang Y, Lin D, Gout PW, editors, Patient-Derived Xenograft Models of Human Cancer. 1st ed. Springer. 2017. p. 11-28 https://doi.org/10.1007/978-3-319-55825-7_2