Toward multiplexing detection of wound healing biomarkers on porous silicon resonant microcavities

Fransiska Sri Herwahyu Krismastuti, Alex Cavallaro, Beatriz Prieto-Simon, Nicolas H. Voelcker

Research output: Contribution to journalArticleResearchpeer-review

14 Citations (Scopus)

Abstract

Bacterial wound infections can cause septicemia and lead to limb amputation or death. Therefore, early detection of bacteria is important in chronic wound management. Here, an optical biosensor based on porous silicon resonant microcavity (pSiRM) structure modified with fluorogenic peptide substrate is demonstrated to detect the presence of Sortase A (SrtA), a bacterial enzyme found in the cell membrane protein of Staphylococcus aureus. The combination of fluorescence enhancement effects of the pSiRM architecture with the incorporation of SrtA fluorogenic peptide substrate within the pSi matrix enables the sensing of SrtA with an outstanding limit of detection of 8 × 10-14 M. Modification of the pSiRM structure with microscale spots of two fluorogenic peptide substrates, one specific for SrtA and the other for matrix metalloproteinases, effectively demonstrates the feasibility to perform multiplexed biomarker analysis. The results in this study highlight the potential of the pSiRM sensing platform as a point-of-care diagnostic tool for biomarkers of bacterial wound infection.

Original languageEnglish
Article number1500383
Number of pages8
JournalAdvanced Science
Volume3
Issue number6
DOIs
Publication statusPublished - Jun 2016
Externally publishedYes

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