Total synthesis of glycosylated human interferon-γ

Xiaoyi Wang; Anneliese S. Ashhurst; Luke J. Dowman; Emma E. Watson; Henry Y. Li; Antony J. Fairbanks; Mark Larance; Ann Kwan; Richard J. Payne

doi:10.1021/acs.orglett.0c02401

Total synthesis of glycosylated human interferon-γ

Xiaoyi Wang, Anneliese S. Ashhurst, Luke J. Dowman, Emma E. Watson, Henry Y. Li, Antony J. Fairbanks, Mark Larance, Ann Kwan, Richard J. Payne

Research output: Contribution to journal › Article › Research › peer-review

23 Citations (Scopus)

Abstract

Interferon-γ(IFN-γ) is a glycoprotein that is responsible for orchestrating numerous critical immune induction and modulation processes and is used clinically for the treatment of a number of diseases. Herein, we describe the total chemical synthesis of homogeneously glycosylated variants of human IFN-γusing a tandem diselenide-selenoester ligation-deselenization strategy in the C- to N-terminal direction. The synthetic glycoproteins were successfully folded, and the structures and antiviral functions were assessed.

Original language	English
Pages (from-to)	6863-6867
Number of pages	5
Journal	Organic Letters
Volume	22
Issue number	17
DOIs	https://doi.org/10.1021/acs.orglett.0c02401
Publication status	Published - 4 Sept 2020
Externally published	Yes

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10.1021/acs.orglett.0c02401

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abstract = "Interferon-γ(IFN-γ) is a glycoprotein that is responsible for orchestrating numerous critical immune induction and modulation processes and is used clinically for the treatment of a number of diseases. Herein, we describe the total chemical synthesis of homogeneously glycosylated variants of human IFN-γusing a tandem diselenide-selenoester ligation-deselenization strategy in the C- to N-terminal direction. The synthetic glycoproteins were successfully folded, and the structures and antiviral functions were assessed.",

author = "Xiaoyi Wang and Ashhurst, {Anneliese S.} and Dowman, {Luke J.} and Watson, {Emma E.} and Li, {Henry Y.} and Fairbanks, {Antony J.} and Mark Larance and Ann Kwan and Payne, {Richard J.}",

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AU - Wang, Xiaoyi

AU - Ashhurst, Anneliese S.

AU - Dowman, Luke J.

AU - Watson, Emma E.

AU - Li, Henry Y.

AU - Fairbanks, Antony J.

AU - Larance, Mark

AU - Kwan, Ann

AU - Payne, Richard J.

PY - 2020/9/4

Y1 - 2020/9/4

N2 - Interferon-γ(IFN-γ) is a glycoprotein that is responsible for orchestrating numerous critical immune induction and modulation processes and is used clinically for the treatment of a number of diseases. Herein, we describe the total chemical synthesis of homogeneously glycosylated variants of human IFN-γusing a tandem diselenide-selenoester ligation-deselenization strategy in the C- to N-terminal direction. The synthetic glycoproteins were successfully folded, and the structures and antiviral functions were assessed.

AB - Interferon-γ(IFN-γ) is a glycoprotein that is responsible for orchestrating numerous critical immune induction and modulation processes and is used clinically for the treatment of a number of diseases. Herein, we describe the total chemical synthesis of homogeneously glycosylated variants of human IFN-γusing a tandem diselenide-selenoester ligation-deselenization strategy in the C- to N-terminal direction. The synthetic glycoproteins were successfully folded, and the structures and antiviral functions were assessed.

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EP - 6867

JO - Organic Letters

JF - Organic Letters

IS - 17

ER -

Total synthesis of glycosylated human interferon-γ

Abstract

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Functional Studies of Tyrosine Sulfation using Synthetic Sulfoproteins

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Total synthesis of glycosylated human interferon-γ

Abstract

UN SDGs

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Functional Studies of Tyrosine Sulfation using Synthetic Sulfoproteins

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