Total chemical synthesis of human activin βA[12-116] and related large-loop polypeptides

Hooi-Hong Keah, Natalie S Allen, Robert Lee Clay, Reinhard I Boysen, Tracy Warner, Milton Thomas William Hearn

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We report here the synthesis, purification, and characterization of several large polypeptides related to the human activin βA subunit and their cyclic counterparts. In particular, we describe for the first time the total chemical synthesis of a 105-mer polypeptide, des[1-11] activin βA, and related large-loop polypeptide, by an optimized solid phase synthetic protocol based on 9-flouroenylmethyoxycarbonyl (Fmoc) chemistry. These studies show that automated chemical synthesis utilizing Fmoc-based solid phase synthetic strategies provides a practical alternative to recombinant DNA technology for the production of activin-related subunits, with the opportunity to rapidly provide different analogues and structural variants for subsequent structure-function and associated biophysical investigations.

Original languageEnglish
Pages (from-to)279-289
Number of pages11
JournalBiopolymers (Peptide Science)
Issue number4
Publication statusPublished - 2001


  • Chemical synthesis
  • ESI-MS
  • Fmoc-solid
  • Human activin β[12-116]
  • Large-loop polypeptides
  • Phase methods

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