Torso-like mediates extracellular accumulation of Furin-cleaved Trunk to pattern the Drosophila embryo termini

Travis Kane Johnson, Michelle Ann Henstridge, Anabel Katrin Herr, Karyn A Moore, James Whisstock, Coral Gay Warr

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Patterning of the Drosophila embryonic termini is achieved by localized activation of the Torso receptor by the growth factor Trunk. Governing this event is the perforin-like protein Torso-like, which is localized to the extracellular space at the embryo poles and has long been proposed to control localized proteolytic activation of Trunk. However, a protease involved in terminal patterning remains to be identified, and the role of Torso-like remains unknown. Here we find that Trunk is cleaved intracellularly by Furin proteases. We further show that Trunk is secreted, and that levels of extracellular Trunk are greatly reduced in torso-like null mutants. On the basis of these and previous findings, we suggest that Torso-like functions to mediate secretion of Trunk, thus providing the mechanism for spatially restricted activation of Torso. Our data represent an alternative mechanism for the spatial control of receptor signalling, and define a different role for perforin-like proteins in eukaryotes
Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalNature Communications
Volume6
DOIs
Publication statusPublished - 2015

Cite this

@article{ef0dcbd07c87453fa17ee562e3e2408b,
title = "Torso-like mediates extracellular accumulation of Furin-cleaved Trunk to pattern the Drosophila embryo termini",
abstract = "Patterning of the Drosophila embryonic termini is achieved by localized activation of the Torso receptor by the growth factor Trunk. Governing this event is the perforin-like protein Torso-like, which is localized to the extracellular space at the embryo poles and has long been proposed to control localized proteolytic activation of Trunk. However, a protease involved in terminal patterning remains to be identified, and the role of Torso-like remains unknown. Here we find that Trunk is cleaved intracellularly by Furin proteases. We further show that Trunk is secreted, and that levels of extracellular Trunk are greatly reduced in torso-like null mutants. On the basis of these and previous findings, we suggest that Torso-like functions to mediate secretion of Trunk, thus providing the mechanism for spatially restricted activation of Torso. Our data represent an alternative mechanism for the spatial control of receptor signalling, and define a different role for perforin-like proteins in eukaryotes",
author = "Johnson, {Travis Kane} and Henstridge, {Michelle Ann} and Herr, {Anabel Katrin} and Moore, {Karyn A} and James Whisstock and Warr, {Coral Gay}",
year = "2015",
doi = "10.1038/ncomms9759",
language = "English",
volume = "6",
pages = "1--6",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",

}

Torso-like mediates extracellular accumulation of Furin-cleaved Trunk to pattern the Drosophila embryo termini. / Johnson, Travis Kane; Henstridge, Michelle Ann; Herr, Anabel Katrin; Moore, Karyn A; Whisstock, James; Warr, Coral Gay.

In: Nature Communications, Vol. 6, 2015, p. 1-6.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Torso-like mediates extracellular accumulation of Furin-cleaved Trunk to pattern the Drosophila embryo termini

AU - Johnson, Travis Kane

AU - Henstridge, Michelle Ann

AU - Herr, Anabel Katrin

AU - Moore, Karyn A

AU - Whisstock, James

AU - Warr, Coral Gay

PY - 2015

Y1 - 2015

N2 - Patterning of the Drosophila embryonic termini is achieved by localized activation of the Torso receptor by the growth factor Trunk. Governing this event is the perforin-like protein Torso-like, which is localized to the extracellular space at the embryo poles and has long been proposed to control localized proteolytic activation of Trunk. However, a protease involved in terminal patterning remains to be identified, and the role of Torso-like remains unknown. Here we find that Trunk is cleaved intracellularly by Furin proteases. We further show that Trunk is secreted, and that levels of extracellular Trunk are greatly reduced in torso-like null mutants. On the basis of these and previous findings, we suggest that Torso-like functions to mediate secretion of Trunk, thus providing the mechanism for spatially restricted activation of Torso. Our data represent an alternative mechanism for the spatial control of receptor signalling, and define a different role for perforin-like proteins in eukaryotes

AB - Patterning of the Drosophila embryonic termini is achieved by localized activation of the Torso receptor by the growth factor Trunk. Governing this event is the perforin-like protein Torso-like, which is localized to the extracellular space at the embryo poles and has long been proposed to control localized proteolytic activation of Trunk. However, a protease involved in terminal patterning remains to be identified, and the role of Torso-like remains unknown. Here we find that Trunk is cleaved intracellularly by Furin proteases. We further show that Trunk is secreted, and that levels of extracellular Trunk are greatly reduced in torso-like null mutants. On the basis of these and previous findings, we suggest that Torso-like functions to mediate secretion of Trunk, thus providing the mechanism for spatially restricted activation of Torso. Our data represent an alternative mechanism for the spatial control of receptor signalling, and define a different role for perforin-like proteins in eukaryotes

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DO - 10.1038/ncomms9759

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JO - Nature Communications

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