Tools for high efficiency genetic manipulation of the human pathogen Penicillium marneffei

Hayley E. Bugeja, Kylie J. Boyce, Harshini Weerasinghe, Sally Beard, Anne Jeziorowski, Shivani Pasricha, Michael Payne, Lena Schreider, Alex Andrianopoulos

Research output: Contribution to journalArticleResearchpeer-review

38 Citations (Scopus)

Abstract

Penicillium marneffei is an opportunistic pathogen of humans and displays a temperature dependent dimorphic transition. Like many fungi, exogenous DNA introduced by DNA mediated transformation is integrated randomly into the genome resulting in inefficient gene deletion and position-specific effects. To enhance successful gene targeting, the consequences of perturbing components of the non-homologous end joining recombination pathway have been examined. The deletion of the KU70 and LIG4 orthologs, pkuA and ligD, respectively, dramatically enhanced the observed homologous recombination frequency leading to efficient gene deletion. While Δ pkuA was associated with reduced genetic stability over-time, Δ ligD represents a suitable recipient strain for downstream applications and combined with a modified Gateway™ system for the rapid generation of gene deletion constructs, this represents an efficient pipeline for characterizing gene function in P. marneffei.

Original languageEnglish
Pages (from-to)772-778
Number of pages7
JournalFungal Genetics and Biology
Volume49
Issue number10
DOIs
Publication statusPublished - Oct 2012
Externally publishedYes

Keywords

  • Dimorphism
  • DNA-mediated transformation
  • Gene targeting
  • Non-homologous end joining
  • Pathogenicity

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