Toll-like receptors: Ligands, cell-based models, and readouts for receptor action

Jennifer K. Dowling, Jérome Dellacasagrande

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

15 Citations (Scopus)

Abstract

This chapter details Toll-like receptors (TLRs) and the tools available to study their biology in vitro. Key parameters to consider before exploring TLR action such as receptor localization, signaling pathways, nature of ligands and cellular expression are introduced. Cellular models (i.e., host cells and readouts) based on the use of cell lines, primary cells, or whole blood are presented. The use of modifi ed TLRs to circumvent some technical problems is also discussed.

Original languageEnglish
Title of host publicationToll-Like Receptors
Subtitle of host publicationPractice and Methods
EditorsClare E McCoy
Place of PublicationNew York USA
PublisherHumana Press
Pages3-27
Number of pages25
Volume1390
Edition2nd
ISBN (Electronic)978-1-4939-3335-8
ISBN (Print)978-1-4939-3333-4
DOIs
Publication statusPublished - 1 Jan 2016

Publication series

NameMethods in Molecular Biology
Volume1390
ISSN (Print)10643745

Keywords

  • Cell-based assays
  • Reporter gene assays
  • TLR ligands
  • Toll-like receptors
  • Whole blood assays

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