TY - JOUR
T1 - Tocotrienols ameliorate neurodegeneration and motor deficits in the 6-ohda-induced rat model of parkinsonism
T2 - Behavioural and immunohistochemistry analysis
AU - Kumari, Mangala
AU - Ramdas, Premdass
AU - Radhakrishnan, Ammu Kutty
AU - Kutty, Methil Kannan
AU - Haleagrahara, Nagaraja
N1 - Funding Information:
Funding: This research was funded by International Medical University (IMU), Research Grant ID: IMU210/2010 and the APC was funded by the Institute for Research, Development, and Innovation (IRDI), IMU.
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021/5/10
Y1 - 2021/5/10
N2 - Parkinson’s disease (PD) is a debilitating neurodegenerative disease, which progresses over time, causing pathological depigmentation of the substantia nigra (SN) in the midbrain due to loss of dopaminergic neurons. Emerging studies revealed the promising effects of some nutrient compounds in reducing the risk of PD. One such nutrient compound that possess neuroprotective effects and prevents neurodegeneration is tocotrienol (T3), a vitamin E family member. In the present study, a single dose intracisternal injection of 250 µg 6-hydroxydopamine (6-OHDA) was used to induce parkinsonism in male Sprague Dawley (SD) rats. Forty-eight hours post injection, the SD rats were orally supplemented with alpha (α)-and gamma (γ)-T3 for 28 days. The neuroprotective effects of α-and γ-T3 were evaluated using behavioural studies and immunohistochemistry (IHC). The findings from this study revealed that supplementation of α-and γ-T3 was able to ameliorate the motor deficits induced by 6-OHDA and improve the neuronal functions by reducing inflammation, reversing the neuronal degradation, and preventing further reduction of dopaminergic neurons in the SN and striatum (STR) fibre density.
AB - Parkinson’s disease (PD) is a debilitating neurodegenerative disease, which progresses over time, causing pathological depigmentation of the substantia nigra (SN) in the midbrain due to loss of dopaminergic neurons. Emerging studies revealed the promising effects of some nutrient compounds in reducing the risk of PD. One such nutrient compound that possess neuroprotective effects and prevents neurodegeneration is tocotrienol (T3), a vitamin E family member. In the present study, a single dose intracisternal injection of 250 µg 6-hydroxydopamine (6-OHDA) was used to induce parkinsonism in male Sprague Dawley (SD) rats. Forty-eight hours post injection, the SD rats were orally supplemented with alpha (α)-and gamma (γ)-T3 for 28 days. The neuroprotective effects of α-and γ-T3 were evaluated using behavioural studies and immunohistochemistry (IHC). The findings from this study revealed that supplementation of α-and γ-T3 was able to ameliorate the motor deficits induced by 6-OHDA and improve the neuronal functions by reducing inflammation, reversing the neuronal degradation, and preventing further reduction of dopaminergic neurons in the SN and striatum (STR) fibre density.
KW - Glial fibrillary acidic protein
KW - Neurofilaments
KW - Neurofunctions
KW - Neuroglia
KW - Parkinson’s disease
KW - Tocotrienols
KW - Tyrosine hydroxylase
KW - Vitamin E
UR - http://www.scopus.com/inward/record.url?scp=85105451328&partnerID=8YFLogxK
U2 - 10.3390/nu13051583
DO - 10.3390/nu13051583
M3 - Article
C2 - 34068460
AN - SCOPUS:85105451328
SN - 2072-6643
VL - 13
JO - Nutrients
JF - Nutrients
IS - 5
M1 - 1583
ER -