Tn6350, a novel transposon carrying pyocin S8 genes encoding a bacteriocin with activity against carbapenemase-producing Pseudomonas aeruginosa

Helena Turano, Fernando Gomes, Gesiele A. Barros-Carvalho, Ralf Lopes, Louise Cerdeira, Luis E.S. Netto, Ana C. Gales, Nilton Lincopan

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3 Citations (Scopus)

Abstract

A novel transposon belonging to the Tn3-like family was identified on the chromosome of a commensal strain of Pseudomonas aeruginosa sequence type 2343 (ET02). Tn6350 is 7,367 bp long and harbors eight open reading frames (ORFs), an ATPase (IS481 family), a transposase (DDE catalytic type), a Tn3 resolvase, three hypothetical proteins, and genes encoding the new pyocin S8 with its immunity protein. We show that pyocin S8 displays activity against carbapenemase-producing P. aeruginosa, including IMP-1, SPM-1, VIM-1, GES-5, and KPC-2 producers.

Original languageEnglish
Article numbere00100
Number of pages4
JournalAntimicrobial Agents and Chemotherapy
Volume61
Issue number5
DOIs
Publication statusPublished - May 2017
Externally publishedYes

Keywords

  • Bacteriocin
  • Metallo-β-lactamases
  • Pseudomonas aeruginosa
  • S-type pyocins
  • Transposon

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