Tissue-wide overexpression of alpha-T-catenin results in aberrant trophoblast invasion but does not cause embryonic mortality in mice

K. Tyberghein, S. Goossens, J. J. Haigh, F. Van Roy, J. Van Hengel

Research output: Contribution to journalArticleResearchpeer-review

7 Citations (Scopus)

Abstract

Transcriptional activation of CTNNA3, encoding αT-catenin, by the Y153H mutated form of the human STOX1 transcription factor was proposed to be responsible for altered fetal trophoblast invasion into the maternal endometrium during placentation in pre-eclampsia. Here we have generated a mouse model to investigate the in vivo effects of ectopic αT-catenin expression on trophoblast invasion. Histological analysis was used to determine the invasive capacities of trophoblasts from transgenic embryos, as well as proliferation rates of spongiotrophoblasts in the junctional zone. Augmented expression of αT-catenin reduced the number of invading trophoblasts but did not cause embryonic mortality. The, αT-catenin positive cells could still invade into the decidual layer and migrated as deeply as wild-type trophoblasts. Furthermore, the junctional zone is enlarged in placentas of mice overexpressing αT-catenin due to hyperproliferation of the residing spongiotrophoblasts, suggesting a pivotal role of αT-catenin levels in the control of the proliferative versus invasive state of trophoblasts during placentation. Our study provides, for the first time, in vivo data on the effects of increased levels of αT-catenin in the placenta. © 2012 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)554-560
Number of pages7
JournalPlacenta
Volume33
Issue number7
DOIs
Publication statusPublished - Jul 2012
Externally publishedYes

Keywords

  • Alpha-T-catenin
  • Junctional zones
  • Pre-eclampsia
  • ROSA locus targeting
  • STOX1
  • Transgenic mice
  • Trophoblast invasion

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