Tissue specificity of cross-reactive allogeneic responses by EBV EBNA3A-specific memory T cells

Lloyd J A D'Orsogna; Dave L Roelen; Ellen MW van der Meer-Prins; Pieter van der Pol; Marry E Franke-van Dijk; Michael Eikmans; Jacqy Anholts; Jamie Rossjohn; James McCluskey; Arend Mulder; Cees van Kooten; Ilias IN Doxiadis; Frans HJ Claas

doi:10.1097/TP.0b013e318207944c

Tissue specificity of cross-reactive allogeneic responses by EBV EBNA3A-specific memory T cells

Lloyd J A D'Orsogna, Dave L Roelen, Ellen MW van der Meer-Prins, Pieter van der Pol, Marry E Franke-van Dijk, Michael Eikmans, Jacqy Anholts, Jamie Rossjohn, James McCluskey, Arend Mulder, Cees van Kooten, Ilias IN Doxiadis, Frans HJ Claas

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › Research › peer-review

31 Citations (Scopus)

Abstract

BACKGROUND: The crossreactivity of Epstein-Barr virus (EBV Epstein-Barr virus nuclear antigen 3A [EBNA3A])-specific CD8 T cells against allogeneic human leukocyte antigen (HLA)-B*44:02 has been shown to be dependent on presentation of self-peptide EEYLQAFTY by the target antigen. In this study, we report that allogeneic HLA-B*44:02 proximal tubular epithelial cells (PTECs) and human umbilical vein endothelial cells (HUVECs) are poor targets for EBV EBNA3A-specific T cells. METHODS: The EEY peptide was exogenously loaded onto HLA-B*44:02 and HLA-B*44:03-expressing PTECs and HUVECs. EEY-peptide-loaded, and unloaded, PTECs and HUVECs were then incubated with serial dilutions of our EBNA3A T-cell clone, in a cytotoxicity assay. RESULTS: Although HLA-B*44:02-expressing PTECs were specifically lysed in proportion to the effector/target ratio by the EBNA3A T-cell clone, without peptide loading, lysis was greatly increased by exogenous EEY peptide loading (15 vs. 75 ; P

Original language	English
Pages (from-to)	494 - 500
Number of pages	7
Journal	Transplantation
Volume	91
Issue number	5
DOIs	https://doi.org/10.1097/TP.0b013e318207944c
Publication status	Published - 2011

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D'Orsogna, L. J. A., Roelen, D. L., van der Meer-Prins, E. MW., van der Pol, P., Franke-van Dijk, M. E., Eikmans, M., Anholts, J., Rossjohn, J., McCluskey, J., Mulder, A., van Kooten, C., Doxiadis, I. IN., & Claas, F. HJ. (2011). Tissue specificity of cross-reactive allogeneic responses by EBV EBNA3A-specific memory T cells. Transplantation, 91(5), 494 - 500. https://doi.org/10.1097/TP.0b013e318207944c

D'Orsogna, Lloyd J A ; Roelen, Dave L ; van der Meer-Prins, Ellen MW ; van der Pol, Pieter ; Franke-van Dijk, Marry E ; Eikmans, Michael ; Anholts, Jacqy ; Rossjohn, Jamie ; McCluskey, James ; Mulder, Arend ; van Kooten, Cees ; Doxiadis, Ilias IN ; Claas, Frans HJ. / Tissue specificity of cross-reactive allogeneic responses by EBV EBNA3A-specific memory T cells. In: Transplantation. 2011 ; Vol. 91, No. 5. pp. 494 - 500.

@article{22be397d29a4494b9632715892e0c399,

title = "Tissue specificity of cross-reactive allogeneic responses by EBV EBNA3A-specific memory T cells",

abstract = "BACKGROUND: The crossreactivity of Epstein-Barr virus (EBV Epstein-Barr virus nuclear antigen 3A [EBNA3A])-specific CD8 T cells against allogeneic human leukocyte antigen (HLA)-B*44:02 has been shown to be dependent on presentation of self-peptide EEYLQAFTY by the target antigen. In this study, we report that allogeneic HLA-B*44:02 proximal tubular epithelial cells (PTECs) and human umbilical vein endothelial cells (HUVECs) are poor targets for EBV EBNA3A-specific T cells. METHODS: The EEY peptide was exogenously loaded onto HLA-B*44:02 and HLA-B*44:03-expressing PTECs and HUVECs. EEY-peptide-loaded, and unloaded, PTECs and HUVECs were then incubated with serial dilutions of our EBNA3A T-cell clone, in a cytotoxicity assay. RESULTS: Although HLA-B*44:02-expressing PTECs were specifically lysed in proportion to the effector/target ratio by the EBNA3A T-cell clone, without peptide loading, lysis was greatly increased by exogenous EEY peptide loading (15 vs. 75 ; P",

author = "D'Orsogna, {Lloyd J A} and Roelen, {Dave L} and {van der Meer-Prins}, {Ellen MW} and {van der Pol}, Pieter and {Franke-van Dijk}, {Marry E} and Michael Eikmans and Jacqy Anholts and Jamie Rossjohn and James McCluskey and Arend Mulder and {van Kooten}, Cees and Doxiadis, {Ilias IN} and Claas, {Frans HJ}",

year = "2011",

doi = "10.1097/TP.0b013e318207944c",

language = "English",

volume = "91",

pages = "494 -- 500",

journal = "Transplantation",

issn = "0041-1337",

publisher = "Lippincott Williams & Wilkins",

number = "5",

}

D'Orsogna, LJA, Roelen, DL, van der Meer-Prins, EMW, van der Pol, P, Franke-van Dijk, ME, Eikmans, M, Anholts, J, Rossjohn, J, McCluskey, J, Mulder, A, van Kooten, C, Doxiadis, IIN & Claas, FHJ 2011, 'Tissue specificity of cross-reactive allogeneic responses by EBV EBNA3A-specific memory T cells', Transplantation, vol. 91, no. 5, pp. 494 - 500. https://doi.org/10.1097/TP.0b013e318207944c

Tissue specificity of cross-reactive allogeneic responses by EBV EBNA3A-specific memory T cells. / D'Orsogna, Lloyd J A; Roelen, Dave L; van der Meer-Prins, Ellen MW; van der Pol, Pieter; Franke-van Dijk, Marry E; Eikmans, Michael; Anholts, Jacqy; Rossjohn, Jamie; McCluskey, James; Mulder, Arend; van Kooten, Cees; Doxiadis, Ilias IN; Claas, Frans HJ.

In: Transplantation, Vol. 91, No. 5, 2011, p. 494 - 500.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - Tissue specificity of cross-reactive allogeneic responses by EBV EBNA3A-specific memory T cells

AU - D'Orsogna, Lloyd J A

AU - Roelen, Dave L

AU - van der Meer-Prins, Ellen MW

AU - van der Pol, Pieter

AU - Franke-van Dijk, Marry E

AU - Eikmans, Michael

AU - Anholts, Jacqy

AU - Rossjohn, Jamie

AU - McCluskey, James

AU - Mulder, Arend

AU - van Kooten, Cees

AU - Doxiadis, Ilias IN

AU - Claas, Frans HJ

PY - 2011

Y1 - 2011

N2 - BACKGROUND: The crossreactivity of Epstein-Barr virus (EBV Epstein-Barr virus nuclear antigen 3A [EBNA3A])-specific CD8 T cells against allogeneic human leukocyte antigen (HLA)-B*44:02 has been shown to be dependent on presentation of self-peptide EEYLQAFTY by the target antigen. In this study, we report that allogeneic HLA-B*44:02 proximal tubular epithelial cells (PTECs) and human umbilical vein endothelial cells (HUVECs) are poor targets for EBV EBNA3A-specific T cells. METHODS: The EEY peptide was exogenously loaded onto HLA-B*44:02 and HLA-B*44:03-expressing PTECs and HUVECs. EEY-peptide-loaded, and unloaded, PTECs and HUVECs were then incubated with serial dilutions of our EBNA3A T-cell clone, in a cytotoxicity assay. RESULTS: Although HLA-B*44:02-expressing PTECs were specifically lysed in proportion to the effector/target ratio by the EBNA3A T-cell clone, without peptide loading, lysis was greatly increased by exogenous EEY peptide loading (15 vs. 75 ; P

AB - BACKGROUND: The crossreactivity of Epstein-Barr virus (EBV Epstein-Barr virus nuclear antigen 3A [EBNA3A])-specific CD8 T cells against allogeneic human leukocyte antigen (HLA)-B*44:02 has been shown to be dependent on presentation of self-peptide EEYLQAFTY by the target antigen. In this study, we report that allogeneic HLA-B*44:02 proximal tubular epithelial cells (PTECs) and human umbilical vein endothelial cells (HUVECs) are poor targets for EBV EBNA3A-specific T cells. METHODS: The EEY peptide was exogenously loaded onto HLA-B*44:02 and HLA-B*44:03-expressing PTECs and HUVECs. EEY-peptide-loaded, and unloaded, PTECs and HUVECs were then incubated with serial dilutions of our EBNA3A T-cell clone, in a cytotoxicity assay. RESULTS: Although HLA-B*44:02-expressing PTECs were specifically lysed in proportion to the effector/target ratio by the EBNA3A T-cell clone, without peptide loading, lysis was greatly increased by exogenous EEY peptide loading (15 vs. 75 ; P

UR - http://www.ncbi.nlm.nih.gov/pubmed/21242884

U2 - 10.1097/TP.0b013e318207944c

DO - 10.1097/TP.0b013e318207944c

M3 - Article

VL - 91

SP - 494

EP - 500

JO - Transplantation

JF - Transplantation

SN - 0041-1337

IS - 5

ER -

Tissue specificity of cross-reactive allogeneic responses by EBV EBNA3A-specific memory T cells

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