Tissue specificity of cross-reactive allogeneic responses by EBV EBNA3A-specific memory T cells

Lloyd J A D'Orsogna, Dave L Roelen, Ellen MW van der Meer-Prins, Pieter van der Pol, Marry E Franke-van Dijk, Michael Eikmans, Jacqy Anholts, Jamie Rossjohn, James McCluskey, Arend Mulder, Cees van Kooten, Ilias IN Doxiadis, Frans HJ Claas

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BACKGROUND: The crossreactivity of Epstein-Barr virus (EBV Epstein-Barr virus nuclear antigen 3A [EBNA3A])-specific CD8 T cells against allogeneic human leukocyte antigen (HLA)-B*44:02 has been shown to be dependent on presentation of self-peptide EEYLQAFTY by the target antigen. In this study, we report that allogeneic HLA-B*44:02 proximal tubular epithelial cells (PTECs) and human umbilical vein endothelial cells (HUVECs) are poor targets for EBV EBNA3A-specific T cells. METHODS: The EEY peptide was exogenously loaded onto HLA-B*44:02 and HLA-B*44:03-expressing PTECs and HUVECs. EEY-peptide-loaded, and unloaded, PTECs and HUVECs were then incubated with serial dilutions of our EBNA3A T-cell clone, in a cytotoxicity assay. RESULTS: Although HLA-B*44:02-expressing PTECs were specifically lysed in proportion to the effector/target ratio by the EBNA3A T-cell clone, without peptide loading, lysis was greatly increased by exogenous EEY peptide loading (15 vs. 75 ; P
Original languageEnglish
Pages (from-to)494 - 500
Number of pages7
Issue number5
Publication statusPublished - 2011

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