Tissue distribution of rat glucagon receptor and GLP-1 receptor gene expression

Jillian L. Dunphy, Russel G. Taylor, Peter J. Fuller

Research output: Contribution to journalArticleResearchpeer-review

112 Citations (Scopus)

Abstract

The regulation of glucose metabolism by glucagon and GLP-1 is well established, but novel functions for these and other proglucagon-derived peptides are less well defined. This paper highlights the diversity of both GLP-1 and glucagon activity by studying the tissue distribution of glucagon and GLP-1 receptor gene expression by both Southern blot analysis of RT-PCR products and nuclease protection assays. By Southern blot analysis of RT-PCR products, GLP-1 receptor mRNA was detected in lung, hypothalamus, hippocampus, cerebral cortex, kidney, pancreas, and throughout the gastrointestinal tract. Glucagon receptor expression was detected in liver, kidney, spleen, thymus, adrenal glands, pancreas, cerebral cortex, lung, and throughout the gastrointestinal tract. Nuclease protection assay revealed glucagon receptor expression to be highest in liver and kidney, whereas GLP-1 receptor expression was only detected by protection assay in lung, stomach, and large bowel. Despite previous evidence that other receptors for proglucagon-derived peptides may exist, no evidence of novel receptors or multiple isoforms of the glucagon and GLP-1 receptors was found, indicating that the two cloned receptors may mediate all the effects of proglucagon-derived peptides, or that novel receptors may share less homology with the glucagon and GLP-1 receptors than previously anticipated.

Original languageEnglish
Pages (from-to)179-186
Number of pages8
JournalMolecular and Cellular Endocrinology
Volume141
Issue number1-2
DOIs
Publication statusPublished - 25 Jun 1998

Keywords

  • GLP-1 receptor
  • Glucagon receptor
  • Intestinal adaptation
  • Nuclease protection assays
  • Proglucagon-derived peptides

Cite this

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Tissue distribution of rat glucagon receptor and GLP-1 receptor gene expression. / Dunphy, Jillian L.; Taylor, Russel G.; Fuller, Peter J.

In: Molecular and Cellular Endocrinology, Vol. 141, No. 1-2, 25.06.1998, p. 179-186.

Research output: Contribution to journalArticleResearchpeer-review

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