Tissue, developmental, and tumor-specific expression of divergent transcripts in Wilms tumor

Annie Huang, Christine E. Campbell, Laura Bonetta, Monica S. McAndrews-Hill, Susan Chilton-MacNeill, Max J. Coppes, David J. Law, Andrew P. Feinberg, Herman Yeger, Bryan R.G. Williams

Research output: Contribution to journalArticleResearchpeer-review

131 Citations (Scopus)

Abstract

The Wilms tumor locus on chromosome 11p13 has been mapped to a region defined by overlapping, tumor-specific deletions. Complementary DNA clones representing transcripts of 2.5 (WIT-1) and 3.5 kb (WIT-2 mapping to this region were isolated from a kidney complementary DNA library. Expression of WIT-1 and WIT-2 was restricted to kidney and spleen. RNase protection revealed divergent transcription of WIT-1 and WIT-2, originating from a DNA region of <600 bp. Both transcripts were present at high concentrations in fetal kidney and at much reduced amounts in 5-year-old and adult kidneys. Eleven of 12 Wilms tumors classified as histopathologically heterogeneous exhibited absent or reduced expression of WIT-2, whereas only 4 of 14 histopathologically homogeneous tumors showed reduced expression. These data demonstrate a molecular basis for the pathogenetic heterogeneity in Wilms tumorigenesis.

Original languageEnglish
Pages (from-to)991-994
Number of pages4
JournalScience
Volume250
Issue number4983
DOIs
Publication statusPublished - 1 Jan 1990
Externally publishedYes

Cite this