TY - JOUR
T1 - Tissue, developmental, and tumor-specific expression of divergent transcripts in Wilms tumor
AU - Huang, Annie
AU - Campbell, Christine E.
AU - Bonetta, Laura
AU - McAndrews-Hill, Monica S.
AU - Chilton-MacNeill, Susan
AU - Coppes, Max J.
AU - Law, David J.
AU - Feinberg, Andrew P.
AU - Yeger, Herman
AU - Williams, Bryan R.G.
PY - 1990/1/1
Y1 - 1990/1/1
N2 - The Wilms tumor locus on chromosome 11p13 has been mapped to a region defined by overlapping, tumor-specific deletions. Complementary DNA clones representing transcripts of 2.5 (WIT-1) and 3.5 kb (WIT-2 mapping to this region were isolated from a kidney complementary DNA library. Expression of WIT-1 and WIT-2 was restricted to kidney and spleen. RNase protection revealed divergent transcription of WIT-1 and WIT-2, originating from a DNA region of <600 bp. Both transcripts were present at high concentrations in fetal kidney and at much reduced amounts in 5-year-old and adult kidneys. Eleven of 12 Wilms tumors classified as histopathologically heterogeneous exhibited absent or reduced expression of WIT-2, whereas only 4 of 14 histopathologically homogeneous tumors showed reduced expression. These data demonstrate a molecular basis for the pathogenetic heterogeneity in Wilms tumorigenesis.
AB - The Wilms tumor locus on chromosome 11p13 has been mapped to a region defined by overlapping, tumor-specific deletions. Complementary DNA clones representing transcripts of 2.5 (WIT-1) and 3.5 kb (WIT-2 mapping to this region were isolated from a kidney complementary DNA library. Expression of WIT-1 and WIT-2 was restricted to kidney and spleen. RNase protection revealed divergent transcription of WIT-1 and WIT-2, originating from a DNA region of <600 bp. Both transcripts were present at high concentrations in fetal kidney and at much reduced amounts in 5-year-old and adult kidneys. Eleven of 12 Wilms tumors classified as histopathologically heterogeneous exhibited absent or reduced expression of WIT-2, whereas only 4 of 14 histopathologically homogeneous tumors showed reduced expression. These data demonstrate a molecular basis for the pathogenetic heterogeneity in Wilms tumorigenesis.
UR - http://www.scopus.com/inward/record.url?scp=0025644455&partnerID=8YFLogxK
U2 - 10.1126/science.2173145
DO - 10.1126/science.2173145
M3 - Article
C2 - 2173145
AN - SCOPUS:0025644455
SN - 0036-8075
VL - 250
SP - 991
EP - 994
JO - Science
JF - Science
IS - 4983
ER -