TINC— A Method to Dissect Regulatory Complexes at Single-Locus Resolution— Reveals an Extensive Protein Complex at the Nanog Promoter

Anja S. Knaupp, Monika Mohenska, Michael R. Larcombe, Ethan Ford, Sue Mei Lim, Kayla Wong, Joseph Chen, Jaber Firas, Cheng Huang, Xiaodong Liu, Trung Nguyen, Yu B.Y. Sun, Melissa L. Holmes, Pratibha Tripathi, Jahnvi Pflueger, Fernando J. Rossello, Jan Schröder, Kathryn C. Davidson, Christian M. Nefzger, Partha P. DasJody J. Haigh, Ryan Lister, Ralf B. Schittenhelm, Jose M. Polo

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10 Citations (Scopus)


Here, Knaupp and colleagues describe TINC, an epigenetic method that allows interrogation of mammalian regulatory complexes at a single-locus resolution. TINC was applied to dissect the transcriptional complex at the Nanog promoter in embryonic stem cells, revealing hundreds of interactors, including RCOR2, hence redefining how this gene is regulated in pluripotency.

Original languageEnglish
Pages (from-to)1246-1259
Number of pages14
JournalStem Cell Reports
Issue number6
Publication statusPublished - 8 Dec 2020


  • Epigenetics
  • iPSCs
  • Nanog
  • pluripotency
  • RCOR2
  • reprogramming
  • single-locus pull-down
  • TINC
  • transcriptional complex

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