TINC— A Method to Dissect Regulatory Complexes at Single-Locus Resolution— Reveals an Extensive Protein Complex at the Nanog Promoter

Anja S. Knaupp; Monika Mohenska; Michael R. Larcombe; Ethan Ford; Sue Mei Lim; Kayla Wong; Joseph Chen; Jaber Firas; Cheng Huang; Xiaodong Liu; Trung Nguyen; Yu B.Y. Sun; Melissa L. Holmes; Pratibha Tripathi; Jahnvi Pflueger; Fernando J. Rossello; Jan Schröder; Kathryn C. Davidson; Christian M. Nefzger; Partha P. Das; Jody J. Haigh; Ryan Lister; Ralf B. Schittenhelm; Jose M. Polo

doi:10.1016/j.stemcr.2020.11.005

TINC— A Method to Dissect Regulatory Complexes at Single-Locus Resolution— Reveals an Extensive Protein Complex at the Nanog Promoter

Anja S. Knaupp, Monika Mohenska, Michael R. Larcombe, Ethan Ford, Sue Mei Lim, Kayla Wong, Joseph Chen, Jaber Firas, Cheng Huang, Xiaodong Liu, Trung Nguyen, Yu B.Y. Sun, Melissa L. Holmes, Pratibha Tripathi, Jahnvi Pflueger, Fernando J. Rossello, Jan Schröder, Kathryn C. Davidson, Christian M. Nefzger, Partha P. DasJody J. Haigh, Ryan Lister, Ralf B. Schittenhelm, Jose M. Polo

Research output: Contribution to journal › Article › Research › peer-review

10 Citations (Scopus)

Abstract

Here, Knaupp and colleagues describe TINC, an epigenetic method that allows interrogation of mammalian regulatory complexes at a single-locus resolution. TINC was applied to dissect the transcriptional complex at the Nanog promoter in embryonic stem cells, revealing hundreds of interactors, including RCOR2, hence redefining how this gene is regulated in pluripotency.

Original language	English
Pages (from-to)	1246-1259
Number of pages	14
Journal	Stem Cell Reports
Volume	15
Issue number	6
DOIs	https://doi.org/10.1016/j.stemcr.2020.11.005
Publication status	Published - 8 Dec 2020

Keywords

Epigenetics
iPSCs
Nanog
pluripotency
RCOR2
reprogramming
single-locus pull-down
TINC
transcriptional complex

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10.1016/j.stemcr.2020.11.005Licence: CC BY-NC-ND

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Knaupp, A. S., Mohenska, M., Larcombe, M. R., Ford, E., Lim, S. M., Wong, K., Chen, J., Firas, J., Huang, C., Liu, X., Nguyen, T., Sun, Y. B. Y., Holmes, M. L., Tripathi, P., Pflueger, J., Rossello, F. J., Schröder, J., Davidson, K. C., Nefzger, C. M., ... Polo, J. M. (2020). TINC— A Method to Dissect Regulatory Complexes at Single-Locus Resolution— Reveals an Extensive Protein Complex at the Nanog Promoter. Stem Cell Reports, 15(6), 1246-1259. https://doi.org/10.1016/j.stemcr.2020.11.005

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title = "TINC— A Method to Dissect Regulatory Complexes at Single-Locus Resolution— Reveals an Extensive Protein Complex at the Nanog Promoter",

abstract = "Here, Knaupp and colleagues describe TINC, an epigenetic method that allows interrogation of mammalian regulatory complexes at a single-locus resolution. TINC was applied to dissect the transcriptional complex at the Nanog promoter in embryonic stem cells, revealing hundreds of interactors, including RCOR2, hence redefining how this gene is regulated in pluripotency.",

keywords = "Epigenetics, iPSCs, Nanog, pluripotency, RCOR2, reprogramming, single-locus pull-down, TINC, transcriptional complex",

author = "Knaupp, {Anja S.} and Monika Mohenska and Larcombe, {Michael R.} and Ethan Ford and Lim, {Sue Mei} and Kayla Wong and Joseph Chen and Jaber Firas and Cheng Huang and Xiaodong Liu and Trung Nguyen and Sun, {Yu B.Y.} and Holmes, {Melissa L.} and Pratibha Tripathi and Jahnvi Pflueger and Rossello, {Fernando J.} and Jan Schr{\"o}der and Davidson, {Kathryn C.} and Nefzger, {Christian M.} and Das, {Partha P.} and Haigh, {Jody J.} and Ryan Lister and Schittenhelm, {Ralf B.} and Polo, {Jose M.}",

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month = dec,

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doi = "10.1016/j.stemcr.2020.11.005",

language = "English",

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Knaupp, AS, Mohenska, M, Larcombe, MR, Ford, E, Lim, SM, Wong, K, Chen, J, Firas, J, Huang, C, Liu, X, Nguyen, T, Sun, YBY, Holmes, ML, Tripathi, P, Pflueger, J, Rossello, FJ, Schröder, J, Davidson, KC, Nefzger, CM, Das, PP, Haigh, JJ, Lister, R, Schittenhelm, RB & Polo, JM 2020, 'TINC— A Method to Dissect Regulatory Complexes at Single-Locus Resolution— Reveals an Extensive Protein Complex at the Nanog Promoter', Stem Cell Reports, vol. 15, no. 6, pp. 1246-1259. https://doi.org/10.1016/j.stemcr.2020.11.005

TY - JOUR

T1 - TINC— A Method to Dissect Regulatory Complexes at Single-Locus Resolution— Reveals an Extensive Protein Complex at the Nanog Promoter

AU - Knaupp, Anja S.

AU - Mohenska, Monika

AU - Larcombe, Michael R.

AU - Ford, Ethan

AU - Lim, Sue Mei

AU - Wong, Kayla

AU - Chen, Joseph

AU - Firas, Jaber

AU - Huang, Cheng

AU - Liu, Xiaodong

AU - Nguyen, Trung

AU - Sun, Yu B.Y.

AU - Holmes, Melissa L.

AU - Tripathi, Pratibha

AU - Pflueger, Jahnvi

AU - Rossello, Fernando J.

AU - Schröder, Jan

AU - Davidson, Kathryn C.

AU - Nefzger, Christian M.

AU - Das, Partha P.

AU - Haigh, Jody J.

AU - Lister, Ryan

AU - Schittenhelm, Ralf B.

AU - Polo, Jose M.

PY - 2020/12/8

Y1 - 2020/12/8

N2 - Here, Knaupp and colleagues describe TINC, an epigenetic method that allows interrogation of mammalian regulatory complexes at a single-locus resolution. TINC was applied to dissect the transcriptional complex at the Nanog promoter in embryonic stem cells, revealing hundreds of interactors, including RCOR2, hence redefining how this gene is regulated in pluripotency.

AB - Here, Knaupp and colleagues describe TINC, an epigenetic method that allows interrogation of mammalian regulatory complexes at a single-locus resolution. TINC was applied to dissect the transcriptional complex at the Nanog promoter in embryonic stem cells, revealing hundreds of interactors, including RCOR2, hence redefining how this gene is regulated in pluripotency.

KW - Epigenetics

KW - iPSCs

KW - Nanog

KW - pluripotency

KW - RCOR2

KW - reprogramming

KW - single-locus pull-down

KW - TINC

KW - transcriptional complex

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DO - 10.1016/j.stemcr.2020.11.005

M3 - Article

C2 - 33296673

AN - SCOPUS:85096959770

SN - 2213-6711

VL - 15

SP - 1246

EP - 1259

JO - Stem Cell Reports

JF - Stem Cell Reports

IS - 6

ER -

TINC— A Method to Dissect Regulatory Complexes at Single-Locus Resolution— Reveals an Extensive Protein Complex at the Nanog Promoter

Abstract

Keywords

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Other files and links

What makes a cell pluripotent?

Monash Proteomics & Metabolomics Facility

Cite this

TINC— A Method to Dissect Regulatory Complexes at Single-Locus Resolution— Reveals an Extensive Protein Complex at the Nanog Promoter

Abstract

Keywords

Access to Document

Other files and links

Projects

What makes a cell pluripotent?

Equipment

Monash Proteomics & Metabolomics Facility

Cite this