TY - JOUR
T1 - Timing of CD8+ T cell responses in relation to commencement of capillary leakage in children with dengue
AU - Dung, Nguyen Thi Phuong
AU - Le Duyen, Huynh Thi
AU - Van Thuy, Nguyen Thi
AU - Van Ngoc, Tran
AU - Vinh Chau, Nguyen Van
AU - Hien, Tran Tinh M
AU - Rowland-Jones, Sarah L
AU - Dong, Tao
AU - Farrar, Jeremy
AU - Wills, Bridget
AU - Simmons, Cameron P.
PY - 2010/6/15
Y1 - 2010/6/15
N2 - Immune activation is a feature of dengue hemorrhagic fever (DHF) and CD8+ T cell responses in particular have been suggested as having a role in the vasculopathy that characterizes this disease. By phenotyping CD8+ T cells (CD38+/HLA-DR+, CD38 +/Ki-67+, or HLA-DR+/Ki-67+) in serial blood samples from children with dengue, we found no evidence of increased CD8+ T cell activation prior to the commencement of resolution of viremia or hemoconcentration. Investigations with MHC class I tetramers to detect NS3133-142-specific CD8+ T cells in two independent cohorts of children suggested the commencement of hemoconcentration and thrombocytopenia in DHF patients generally begins before the appearance of measurable frequencies of NS3133-142-specific CD8+ T cells. The temporal mismatch between the appearance of measurable surface activated or NS3133-142-specific CD8+ T cells suggests that these cells are sequestered at sites of infection, have phenotypes not detected by our approach, or that other mechanisms independent of CD8+ T cells are responsible for early triggering of capillary leakage in children with DHF.
AB - Immune activation is a feature of dengue hemorrhagic fever (DHF) and CD8+ T cell responses in particular have been suggested as having a role in the vasculopathy that characterizes this disease. By phenotyping CD8+ T cells (CD38+/HLA-DR+, CD38 +/Ki-67+, or HLA-DR+/Ki-67+) in serial blood samples from children with dengue, we found no evidence of increased CD8+ T cell activation prior to the commencement of resolution of viremia or hemoconcentration. Investigations with MHC class I tetramers to detect NS3133-142-specific CD8+ T cells in two independent cohorts of children suggested the commencement of hemoconcentration and thrombocytopenia in DHF patients generally begins before the appearance of measurable frequencies of NS3133-142-specific CD8+ T cells. The temporal mismatch between the appearance of measurable surface activated or NS3133-142-specific CD8+ T cells suggests that these cells are sequestered at sites of infection, have phenotypes not detected by our approach, or that other mechanisms independent of CD8+ T cells are responsible for early triggering of capillary leakage in children with DHF.
UR - http://www.scopus.com/inward/record.url?scp=77953646070&partnerID=8YFLogxK
U2 - 10.4049/jimmunol.0903262
DO - 10.4049/jimmunol.0903262
M3 - Article
AN - SCOPUS:77953646070
SN - 0022-1767
VL - 184
SP - 7281
EP - 7287
JO - Journal of Immunology
JF - Journal of Immunology
IS - 12
ER -