Timing of CD8+ T cell responses in relation to commencement of capillary leakage in children with dengue

Nguyen Thi Phuong Dung, Huynh Thi Le Duyen, Nguyen Thi Van Thuy, Tran Van Ngoc, Nguyen Van Vinh Chau, Tran Tinh M Hien, Sarah L Rowland-Jones, Tao Dong, Jeremy Farrar, Bridget Wills, Cameron P. Simmons

Research output: Contribution to journalArticleResearchpeer-review

66 Citations (Scopus)

Abstract

Immune activation is a feature of dengue hemorrhagic fever (DHF) and CD8+ T cell responses in particular have been suggested as having a role in the vasculopathy that characterizes this disease. By phenotyping CD8+ T cells (CD38+/HLA-DR+, CD38 +/Ki-67+, or HLA-DR+/Ki-67+) in serial blood samples from children with dengue, we found no evidence of increased CD8+ T cell activation prior to the commencement of resolution of viremia or hemoconcentration. Investigations with MHC class I tetramers to detect NS3133-142-specific CD8+ T cells in two independent cohorts of children suggested the commencement of hemoconcentration and thrombocytopenia in DHF patients generally begins before the appearance of measurable frequencies of NS3133-142-specific CD8+ T cells. The temporal mismatch between the appearance of measurable surface activated or NS3133-142-specific CD8+ T cells suggests that these cells are sequestered at sites of infection, have phenotypes not detected by our approach, or that other mechanisms independent of CD8+ T cells are responsible for early triggering of capillary leakage in children with DHF.

Original languageEnglish
Pages (from-to)7281-7287
Number of pages7
JournalJournal of Immunology
Volume184
Issue number12
DOIs
Publication statusPublished - 15 Jun 2010
Externally publishedYes

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