The Larder Lake - Cadillac deformation zone is one of several anastomosing zones of high strain within the Abitibi greenstone belt. In the Kirkland Lake area, Ontario, the Larder Lake - Cadillac deformation zone is characterized by extensive carbonate and chlorite alteration, strong south-dipping foliations, and steep lineations. These features formed during two ductile deformation increments, D2 and D3, that occurred after deposition of Timiskaming assemblage sediments. D2 strain accumulation and greenschist facies metamorphism and alteration were localized within the deformation zone, facilitated by channelling of hydrothermal fluids within a preexisting structure, possibly formed during early D1 terrane accretion. During D2 north-south shortening, east-west-trending sectors of the deformation zone accumulated bulk coaxial strains, while southeast- and northeast-trending sectors experienced, respectively, dextral and sinistral transpressive deformations. Preservation of Timiskaming assemblage sediments in the footwall of the deformation zone indicates a component of south-over-north (reverse) displacement that is not recorded by D2 fabrics. Northwest-southeast D3 compression resulted in the formation of a regional, northeast-striking cleavage formed under regional greenschist facies conditions, and local dextral reactivation of suitably oriented sections of the Larder Lake - Cadillac deformation zone. The Murdoch Creek and Lebel stocks abut the Larder Lake - Cadillac deformation zone. Their internal structure and emplacement are interpreted to be a consequence of D2 north-south shortening. Magmatic zircon and titanite in the Murdoch Creek and Lebel stocks yield U-Pb geochronology ages of 2672 ± 2 and 2673 ± 2 Ma, providing a maximum age for D2 deformation. Hydrothermal titantite associated with S3 foliation in the Murdoch Creek stock gives an U-Pb age of 2665 ± 4 Ma, the maximum age of D3 deformation. Pluton emplacement, deformation, and coincident metamorphism occurred over a span of 1 Ma (from 2670 to 2669 Ma) to over 14 Ma (from 2675 to 2661 Ma), during a regime of north-south, followed by northwest-southeast, regional shortening.