Thymospheres are formed by mesenchymal cells with the potential to generate adipocytes, but not epithelial cells

Julie M. Sheridan, Ashleigh Keown, Antonia Policheni, Siti N.A. Roesley, Noa Rivlin, Matthew E. Ritchie, Reema Jain, Jakub Abramson, Tracy S.P. Heng, Daniel H.D. Gray

Research output: Contribution to journalArticleResearchpeer-review

9 Citations (Scopus)

Abstract

Evidence suggests that a stem-cell-driven differentiation hierarchy maintains the dynamic thymic epithelial cell (TEC) network that governs T lymphocyte development. The identification of TEC stem/progenitor cells has been a major focus in the field, and several candidates with contrasting phenotypes have been described. We sought to determine the provenance and function of the only population reported to exhibit TEC stem cell properties in the adult, a Foxn1− EpCAM− cell that generates so-called thymospheres. We provide evidence that the thymosphere-forming cell (TSFC) is not a TEC stem cell but can incorporate bystander TECs into thymospheres, providing an explanation for the epithelial activity ascribed to these structures. TSFCs were found to share a phenotype, transcriptional profile, and developmental origin with thymic fibroblasts and can generate adipocytes. In summary, this study redefines the nature of bipotent TEC stem/progenitor cells in the adult thymus and highlights a potentially important mesenchymal progenitor population.
Original languageEnglish
Pages (from-to)934-942
Number of pages9
JournalCell Reports
Volume21
Issue number4
DOIs
Publication statusPublished - 24 Oct 2017

Keywords

  • thymus
  • mesenchymal progenitor cell
  • thymic epithelial cell

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