Thymic plasmacytoid dendritic cells are susceptible to productive HIV-1 infection and efficiently transfer R5 HIV-1 to thymocytes in vitro

Vanessa Evans, Luxshimi Lal, Ramesh Akkina, Ajantha Solomon, Edwina Wright, Sharon Lewin, Paul Cameron

Research output: Contribution to journalArticleResearchpeer-review

21 Citations (Scopus)

Abstract

HIV-1 infection of the thymus contributes to the defective regeneration and loss of CD4+ T cells in HIV-1-infected individuals. As thymic dendritic cells (DC) are permissive to infection by HIV-1, we examined the ability of thymic DC to enhance infection of thymocytes which may contribute to the overall depletion of CD4+ T cells. We compared productive infection in isolated human thymic and blood CD11c+ myeloid DC (mDC) and CD123+ plasmacytoid DC (pDC) using enhanced green fluorescent protein (EGFP) CCR5 (R5)-tropic NL(AD8) and CXCR4 (X4)-tropic NL4-3 HIV-1 reporter viruses. Transfer of productive HIV-1 infection from thymic mDC and pDC was determined by culturing these DC subsets either alone or with sorted thymocytes
Original languageEnglish
Pages (from-to)1 - 12
Number of pages12
JournalRetrovirology
Volume8
Issue number43
DOIs
Publication statusPublished - 2011

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