Thymic changes after chorioamnionitis induced by intraamniotic lipopolysaccharide in fetal sheep

Steffen Kunzmann, Kerstin Glogger, Jasper V Been, Suhas G Kallapur, Ilias Nitsos, Timothy James Murugesan Moss, Christian P Speer, John P Newnham, Alan H Jobe, Boris W Kramer

Research output: Contribution to journalArticleResearchpeer-review

Abstract

OBJECTIVE: Regulatory T lymphocytes mediate homeostasis of the immune system and differentiate under the control of the transcription factor FoxP3 in the fetal thymus. We asked whether fetal inflammation caused by chorioamnionitis would modulate thymus development. STUDY DESIGN: Fetal sheep were exposed to an intraamniotic injection of 10 mg lipopolysaccharide at 5 hours, 1 day, 2 days, or 5 days before delivery at 123 gestation days. Cord blood lymphocytes, plasma cortisol, and thymus weight were measured. Glucocorticoid receptor-, activated caspase-3-, Ki-67-, proliferating cell nuclear antigen-, nuclear factor-kappaB-, and FoxP3-positive cells were immunohistochemically evaluated in thymus. RESULTS: Intraamniotic lipopolysaccharide exposure decreased the number of circulating lymphocytes by 40 after 1 day. Thymus-to-body weight ratios were reduced in all lipopolysaccharide groups by a maximum of 40 at 5 days. Lipopolysaccharide exposure modestly increased plasma cortisol concentration, increased nuclear factor-kappaB immunostaining in fetal thymus and reduced the number of FoxP3-positive cells by 40 at 1 day. CONCLUSION: Intraamniotic exposure to lipopolysaccharide induced thymic changes and influenced thymic FoxP3 expression.
Original languageEnglish
Pages (from-to)476.e1 - 476.e9
Number of pages9
JournalAmerican Journal of Obstetrics and Gynecology
Volume202
Issue number5
DOIs
Publication statusPublished - 2010

Cite this

Kunzmann, Steffen ; Glogger, Kerstin ; Been, Jasper V ; Kallapur, Suhas G ; Nitsos, Ilias ; Moss, Timothy James Murugesan ; Speer, Christian P ; Newnham, John P ; Jobe, Alan H ; Kramer, Boris W. / Thymic changes after chorioamnionitis induced by intraamniotic lipopolysaccharide in fetal sheep. In: American Journal of Obstetrics and Gynecology. 2010 ; Vol. 202, No. 5. pp. 476.e1 - 476.e9.
@article{2ed1a94096cb44a980a04e425c36ee33,
title = "Thymic changes after chorioamnionitis induced by intraamniotic lipopolysaccharide in fetal sheep",
abstract = "OBJECTIVE: Regulatory T lymphocytes mediate homeostasis of the immune system and differentiate under the control of the transcription factor FoxP3 in the fetal thymus. We asked whether fetal inflammation caused by chorioamnionitis would modulate thymus development. STUDY DESIGN: Fetal sheep were exposed to an intraamniotic injection of 10 mg lipopolysaccharide at 5 hours, 1 day, 2 days, or 5 days before delivery at 123 gestation days. Cord blood lymphocytes, plasma cortisol, and thymus weight were measured. Glucocorticoid receptor-, activated caspase-3-, Ki-67-, proliferating cell nuclear antigen-, nuclear factor-kappaB-, and FoxP3-positive cells were immunohistochemically evaluated in thymus. RESULTS: Intraamniotic lipopolysaccharide exposure decreased the number of circulating lymphocytes by 40 after 1 day. Thymus-to-body weight ratios were reduced in all lipopolysaccharide groups by a maximum of 40 at 5 days. Lipopolysaccharide exposure modestly increased plasma cortisol concentration, increased nuclear factor-kappaB immunostaining in fetal thymus and reduced the number of FoxP3-positive cells by 40 at 1 day. CONCLUSION: Intraamniotic exposure to lipopolysaccharide induced thymic changes and influenced thymic FoxP3 expression.",
author = "Steffen Kunzmann and Kerstin Glogger and Been, {Jasper V} and Kallapur, {Suhas G} and Ilias Nitsos and Moss, {Timothy James Murugesan} and Speer, {Christian P} and Newnham, {John P} and Jobe, {Alan H} and Kramer, {Boris W}",
year = "2010",
doi = "10.1016/j.ajog.2010.02.035",
language = "English",
volume = "202",
pages = "476.e1 -- 476.e9",
journal = "American Journal of Obstetrics and Gynecology",
issn = "0002-9378",
publisher = "Elsevier",
number = "5",

}

Kunzmann, S, Glogger, K, Been, JV, Kallapur, SG, Nitsos, I, Moss, TJM, Speer, CP, Newnham, JP, Jobe, AH & Kramer, BW 2010, 'Thymic changes after chorioamnionitis induced by intraamniotic lipopolysaccharide in fetal sheep' American Journal of Obstetrics and Gynecology, vol. 202, no. 5, pp. 476.e1 - 476.e9. https://doi.org/10.1016/j.ajog.2010.02.035

Thymic changes after chorioamnionitis induced by intraamniotic lipopolysaccharide in fetal sheep. / Kunzmann, Steffen; Glogger, Kerstin; Been, Jasper V; Kallapur, Suhas G; Nitsos, Ilias; Moss, Timothy James Murugesan; Speer, Christian P; Newnham, John P; Jobe, Alan H; Kramer, Boris W.

In: American Journal of Obstetrics and Gynecology, Vol. 202, No. 5, 2010, p. 476.e1 - 476.e9.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Thymic changes after chorioamnionitis induced by intraamniotic lipopolysaccharide in fetal sheep

AU - Kunzmann, Steffen

AU - Glogger, Kerstin

AU - Been, Jasper V

AU - Kallapur, Suhas G

AU - Nitsos, Ilias

AU - Moss, Timothy James Murugesan

AU - Speer, Christian P

AU - Newnham, John P

AU - Jobe, Alan H

AU - Kramer, Boris W

PY - 2010

Y1 - 2010

N2 - OBJECTIVE: Regulatory T lymphocytes mediate homeostasis of the immune system and differentiate under the control of the transcription factor FoxP3 in the fetal thymus. We asked whether fetal inflammation caused by chorioamnionitis would modulate thymus development. STUDY DESIGN: Fetal sheep were exposed to an intraamniotic injection of 10 mg lipopolysaccharide at 5 hours, 1 day, 2 days, or 5 days before delivery at 123 gestation days. Cord blood lymphocytes, plasma cortisol, and thymus weight were measured. Glucocorticoid receptor-, activated caspase-3-, Ki-67-, proliferating cell nuclear antigen-, nuclear factor-kappaB-, and FoxP3-positive cells were immunohistochemically evaluated in thymus. RESULTS: Intraamniotic lipopolysaccharide exposure decreased the number of circulating lymphocytes by 40 after 1 day. Thymus-to-body weight ratios were reduced in all lipopolysaccharide groups by a maximum of 40 at 5 days. Lipopolysaccharide exposure modestly increased plasma cortisol concentration, increased nuclear factor-kappaB immunostaining in fetal thymus and reduced the number of FoxP3-positive cells by 40 at 1 day. CONCLUSION: Intraamniotic exposure to lipopolysaccharide induced thymic changes and influenced thymic FoxP3 expression.

AB - OBJECTIVE: Regulatory T lymphocytes mediate homeostasis of the immune system and differentiate under the control of the transcription factor FoxP3 in the fetal thymus. We asked whether fetal inflammation caused by chorioamnionitis would modulate thymus development. STUDY DESIGN: Fetal sheep were exposed to an intraamniotic injection of 10 mg lipopolysaccharide at 5 hours, 1 day, 2 days, or 5 days before delivery at 123 gestation days. Cord blood lymphocytes, plasma cortisol, and thymus weight were measured. Glucocorticoid receptor-, activated caspase-3-, Ki-67-, proliferating cell nuclear antigen-, nuclear factor-kappaB-, and FoxP3-positive cells were immunohistochemically evaluated in thymus. RESULTS: Intraamniotic lipopolysaccharide exposure decreased the number of circulating lymphocytes by 40 after 1 day. Thymus-to-body weight ratios were reduced in all lipopolysaccharide groups by a maximum of 40 at 5 days. Lipopolysaccharide exposure modestly increased plasma cortisol concentration, increased nuclear factor-kappaB immunostaining in fetal thymus and reduced the number of FoxP3-positive cells by 40 at 1 day. CONCLUSION: Intraamniotic exposure to lipopolysaccharide induced thymic changes and influenced thymic FoxP3 expression.

UR - http://www.sciencedirect.com/science/article/pii/S0002937810002486

U2 - 10.1016/j.ajog.2010.02.035

DO - 10.1016/j.ajog.2010.02.035

M3 - Article

VL - 202

SP - 476.e1 - 476.e9

JO - American Journal of Obstetrics and Gynecology

JF - American Journal of Obstetrics and Gynecology

SN - 0002-9378

IS - 5

ER -