Thrombospondin-1 expression and localization in the developing ovine lung

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Abstract

Fetal lung growth is critically dependent on the degree to which the lungs are expanded by liquid, although the mechanisms involved are unknown. As thrombospondin-1 (TSP-1) can regulate cell proliferation, attachment, spreading and angiogenesis, we investigated the effects of alterations in fetal lung expansion on TSP-1 expression in sheep. TSP-1 mRNA levels were investigated using Northern blot analysis and in situ hybridisation, whereas the protein levels were determined by immunohistochemistry. Early growth response 1 (EGR1) mRNA levels were measured by quantitative real-time PCR. TSP-1 was expressed in type-II alveolar epithelial cells and fibroblasts and its mRNA levels increased from 100.0+/-14.0 in control fetuses to 347.5+/-73.6 at 36h of increased lung expansion (p
Original languageEnglish
Pages (from-to)625 - 635
Number of pages11
JournalThe Journal of Physiology
Volume584
Issue numberPt 2
Publication statusPublished - 2007

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