Three-dimensional in vitro culture techniques for mesenchymal stem cells

Fatima A. Saleh, Jessica E. Frith, Jennifer A. Lee, Paul G Genever

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

21 Citations (Scopus)


In recent years there has been a growing interest in culturing adherent cells using three-dimensional (3D) techniques, rather than more conventional 2D culture methods. This interest emerges from the realization that growing cells on plastic surfaces cannot truly re-create 3D in vivo conditions and therefore might be limiting the cells' potential. In addition, adult stem cells exist in specialized microenvironments, or niches, where the spatial organization of different niche elements (such as different cell types, extracellular matrix) contributes significantly to stem cell maintenance, which cannot be represented using 2D in vitro models. We have generated a range of different 3D approaches for the analysis of mesenchymal stem cells (MSCs) using both mono- and co-culture environments.

Original languageEnglish
Title of host publicationProgenitor Cells
Subtitle of host publicationMethods and Protocols
EditorsKimberly A. Mace, Kristin M. Braun
Place of PublicationNew York NY USA
Number of pages15
ISBN (Electronic)9781617799808
ISBN (Print)9781617799792
Publication statusPublished - 2012
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Cell tracking
  • Co-culture
  • MSC Spheroids
  • Rotating wall vessel
  • Spinner flask

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