Thermostability and reversibility of silver nanoparticle-protein binding

Bo Wang, Shane A Seabrook, Praveen Nedumpully-Govindan, Pengyu Chen, Hong Yin, Lynne J Waddington, Vidana Chandana Epa, David Alan Winkler, Jason K Kirby, Feng Ding, Pu Chun Ke

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25 Citations (Scopus)

Abstract

The interactions between nanoparticles (NPs) and proteins in living systems are a precursor to the formation of a NP-protein corona that underlies cellular and organism responses to nanomaterials. However, the thermodynamic properties and reversibility of NP-protein interactions have rarely been examined. Using an automated, high-throughput and temperature-controlled dynamic light scattering (DLS) technique we observed a distinct hysteresis in the hydrodynamic radius of branched polyethyleneimine (BPEI) coated-silver nanoparticles (bAgNPs) exposed to like-charged lysozyme during the processes of heating and cooling, in contrast to the irreversible interactions between bAgNPs and oppositely charged alpha lactalbumin (ALact). Our discrete molecular dynamics (DMD) simulations offered a new molecular insight into the differential structure, dynamics and thermodynamics of bAgNPs binding with the two protein homologs and further revealed the different roles of the capping agents of citrate and BPEI in NP-protein interactions. This study facilitates our understanding of the transformation of nanomaterials in living systems, whose implications range from the field study of nanotoxicology to nanomaterials synthesis, nanobiotechnology and nanomedicine.
Original languageEnglish
Pages (from-to)1728-1739
Number of pages12
JournalPhysical Chemistry Chemical Physics
Volume17
Issue number3
DOIs
Publication statusPublished - 2015

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