Therapeutic potency of nanoformulations of siRNAs and shRNAs in animal models of cancers

Md Emranul Karim, Kyi Kyi Tha, Iekhsan Othman, Mohammad Borhan Uddin, Ezharul Hoque Chowdhury

    Research output: Contribution to journalReview ArticleResearchpeer-review

    12 Citations (Scopus)

    Abstract

    RNA Interference (RNAi) has brought revolutionary transformations in cancer management in the past two decades. RNAi-based therapeutics including siRNA and shRNA have immense scope to silence the expression ofmutant cancer genes specifically in a therapeutic context. Although tremendous progress has been made to establish catalytic RNA as a new class of biologics for cancer management, a lot of extracellular and intracellular barriers still pose a long-lasting challenge on the way to clinical approval. A series of chemically suitable, safe and effective viral and non-viral carriers have emerged to overcome physiological barriers and ensure targeted delivery of RNAi. The newly invented carriers, delivery techniques and gene editing technology made current treatment protocols stronger to fight cancer. This review has provided a platform about the chronicle of siRNA development and challenges of RNAi therapeutics for laboratory to bedside translation focusing on recent advancement in siRNA delivery vehicles with their limitations. Furthermore, an overview of several animal model studies of siRNA- or shRNA-based cancer gene therapy over the past 15 years has been presented, highlighting the roles of genes in multiple cancers, pharmacokinetic parameters and critical evaluation. The review concludes with a future direction for the development of catalytic RNA vehicles and design strategies to make RNAi-based cancer gene therapy more promising to surmount cancer gene delivery challenges.

    Original languageEnglish
    Article number65
    Number of pages44
    JournalPharmaceutics
    Volume10
    Issue number2
    DOIs
    Publication statusPublished - 26 May 2018

    Keywords

    • Cancer
    • Gene therapy
    • RNA interference
    • Short hairpin RNA(shrna)
    • Small interfering RNA(sirna)
    • Viral and non-viral carriers

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